Multiplex Assay Kit for Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay) Mus musculus (Mouse), Rattus norvegicus (Rat) Multiplex ELISA

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA946Mi.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-89 86
EDTA plasma(n=5) 96-105 101
heparin plasma(n=5) 79-90 78

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-95% 98-105% 88-101% 78-104%
EDTA plasma(n=5) 78-89% 89-102% 78-93% 86-99%
heparin plasma(n=5) 85-99% 93-102% 90-98% 90-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:Ab1-42) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.

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Citations

  • Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s DiseasePubmed: 23493042
  • The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1–42 seeding and short-term memory deficits in the Aβ25–35 mouse model of Alzheimer's diseasePubmed: 23123349
  • 慢病毒介导的ＣＢＳ基因过表达对原代皮质神经元Globesci:Source
  • 外源性硫化氢对高糖条件下原代神经元β淀粉样蛋白的影响J-Smu:Source
  • Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat BrainPubmed:24792732
  • Amyloid ? lowering and cognition enhancing effects of ghrelin receptor analog [D-Lys (3)] GHRP-6 in rat model of obesityPubmed:25296496
  • Anesthetic Propofol Attenuates Apoptosis, Aβ Accumulation, and Inflammation Induced by Sevoflurane Through NF-κB Pathway in Human Neuroglioma CellsPubMed: 25809614
  • Autophagy dysfunction upregulates beta-amyloid peptides via enhancing the activity of γ-secretase complexPubMed: 26316755
  • Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS2 transgenic model of Alzheimer&#039;s diseasePubMed: 25882166
  • The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Ratsopen-access:
  • 芝麻素对高糖条件下PC12细胞Aβ和BACE-1表达的影响article:10299
  • Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's DiseasePubmed:26993489
  • ОСОБЕННОСТИ ПРОЦЕССОВ ФОРМИРОВАНИЯ ПОКАЗАТЕЛЕЙ ФУНКЦИОНАЛЬНОГО СОСТОЯНИЯ ВЫСШЕЙ НЕРВНОЙ ДЕЯТЕЛЬНОСТИ И ХАРАКТЕРИСТИКИ ВНИМАНИЯ СТУДЕНТОВSMB-2016-03-062.pdf
  • 芝麻素对高糖条件下PC12 细胞Aß 和BACE 1 表达的影响QK:92621A
  • Geniposide attenuates the level of Aβ1–42 via enhancing leptin signaling in cellular and APP/PS1 transgenic micepubmed:28160136
  • Effects of dietary glucocerebrosides from sea cucumber on the brain sphingolipid profiles of mouse models of Alzheimer's disease.pubmed:28232989
  • Norovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in a mouse model of Alzheimer’s diseasearticles:srep41041.ris
  • Cerebrosides from Sea Cucumber Protect Against Oxidative Stress in SAMP8 Mice and PC12 Cellspubmed:28406733
  • Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat dietPubmed:29986309
  • Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female ratsPubmed:30028991
  • Sub-chronic celecoxib prevents soluble beta amyloid-induced depressive-like behaviour in ratsPubmed:29879605
  • Punicalagin ameliorates the elevation of plasma homocysteine, amyloid-β, TNF-α and apoptosis by advocating antioxidants and modulating apoptotic mediator proteins in brain modulating apoptotic mediator …Pubmed:29579708
  • Effects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic MicePubmed:29695154
  • Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in ratsPubmed:29522778
  • CART peptide activates the Nrf2/HO-1 antioxidant pathway and protects hippocampal neurons in a rat model of Alzheimer's diseasePubmed:29777699
  • Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse …Pubmed:29731975
  • Ellagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β‑amyloid production and tau hyperphosphorylationDoi: 10.3892/etm.2018.6860
  • 非编码RNA miR-137 调控tau 蛋白磷酸化和L 型钙离子通道CACNA1C 参与阿尔茨海默病的发生
  • (台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探
  • The Efficacy of Sauropus androgynus Leaves Extract To Improve Cognitive Function in Wistar Rats Induced Alzheimer's
  • Effect of Aluminum-Maltolate on the Content of Aβ Protein and the Expression of ApoER2, VLDLRs, and LRP1 in PC12-ApoE4 CellsPubmed: 30649678
  • Imbalance of Microglial TLR4/TREM2 in LPS-Treated APP/PS1 Transgenic Mice: A Potential Link Between Alzheimer's Disease and Systemic InflammationPubmed: 30756214
  • Repetitive transcranial magnetic stimulation protects mice against 6-OHDA-induced Parkinson's disease symptoms by regulating brain amyloid β level
  • Obstructive sleep apnea may increase the risk of Alzheimer's diseasePubmed: 31487291
  • Nanoformulated ellagic acid ameliorates pentylenetetrazol-induced experimental epileptic seizures by modulating oxidative stress, inflammatory cytokines and …Pubmed: 31728888
  • Neurobiological determinants of depressive-like symptoms in rodents
  • Mahanimbine-induced neuroprotection via cholinergic system and attenuated amyloidogenesis as well as neuroinflammation in lipopolysaccharides-induced …
  • Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet.
  • Ex Vivo Investigation of Bexarotene and Nicotinamide Function as a Protect?ve Agent on Rat Synaptosomes Treated with A¦Â (1-42)33428094
  • Comparative study on the effects of phosphatidylserine rich in DHA and EPA on A¦Â-intervened Alzheimer's disease using cell models33876786
  • Protective effects of repetitive transcranial magnetic stimulation against 6-OHDA-induced Parkinson's symptoms in a mice model: the key role of miR-409-3p/PDHB ¡­34078216
  • Immunization with A¦Â3-10-KLH vaccine improves cognitive function and ameliorates mitochondrial dysfunction and reduces Alzheimer's disease-like pathology in Tg ¡­34044034
  • The effect of a 6-month Ketogenic Medium-Chain Triglyceride Supplement on Plasma Cardiometabolic and Inflammatory Markers in Mild Cognitive?¡­33906081
  • Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent)
  • GPNMB mitigates Alzheimer's disease and enhances autophagy via suppressing the mTOR signal34695452
  • The influence of vinpocetine alone or in combination with Epigallocatechin-3-gallate, Coenzyme COQ10, Vitamin E and Selenium as a potential neuroprotective …34706318
  • Probiotics ameliorates LPS induced neuroinflammation injury on Aβ 1–42, APP, γ-β secretase and BDNF levels in maternal gut microbiota and fetal …Pubmed:35312928
  • New Thienopyridine Scaffold as An Antidementia Lead: In Silico Study and Biological Screening

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