Multiplex Assay Kit for Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay) Chicken (Gallus) Multiplex ELISA

ANG1; AGP1; AGPT; ANG-I

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA008Ga.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-98 86
EDTA plasma(n=5) 89-101 95
heparin plasma(n=5) 79-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-105% 79-89% 78-102% 86-101%
EDTA plasma(n=5) 93-101% 78-93% 82-95% 79-97%
heparin plasma(n=5) 88-97% 95-105% 79-96% 90-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:ANGPT1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Angiopoietin 1 (ANGPT1) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in EwesScience: Article
  • High Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial InfarctionPubMed: 25735431
  • Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu
  • Angiopoietin-1 facilitates recovery of hematopoiesis in radiated micePubmed:27347310
  • Increased plasma concentrations of interleukin 35 in patients with coronary artery diseaseJournal:-19
  • A new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancerpubmed:28425270
  • Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis.pubmed:28276491
  • The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarkerpubmed:28584715
  • Prediction of Response to Sorafenib in Hepatocellular Carcinoma: A Marker Panel by Multiple Reaction Monitoring-Mass Spectrometrypubmed:28550167
  • Cervical cancer cell–derived angiopoietins promote tumor progressionpubmed:28720059
  • RHEUMATOID ARTHRITIS RELATED BIOMARKER20170350884
  • Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in …Pubmed:28968277
  • Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats10.1016:j.bja.2018.05.049
  • Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in micePubmed:29929978
  • Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor RelapseDoi: 10.4143/crt.2018.214
  • Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factorsPubmed: 30929911
  • Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep ApneaPubmed: 31882827
  • Effects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytesPubmed: 32800538
  • Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells33705953
  • The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats33997943
  • Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study34282706
  • RAR-Related Orphan Receptor: An Accelerated Preeclampsia Progression by Activating the JAK/STAT3 PathwayPubmed:35619579

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