Multiplex Assay Kit for Apolipoprotein E (APOE) ,etc. by FLIA (Flow Luminescence Immunoassay)
Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 449.00 US$ 467.00 US$ 492.00 US$ 527.00 US$ 562.00 US$ 613.00 US$ 691.00 US$ 864.00
- Quantity
Overview
Properties
- Product No.LMA704Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayCardiovascular biologyHepatology
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Recovery
Matrices listed below were spiked with certain level of recombinant Apolipoprotein E (APOE) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein E (APOE) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 87-101 | 91 |
EDTA plasma(n=5) | 80-99 | 83 |
heparin plasma(n=5) | 78-95 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein E (APOE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein E (APOE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein E (APOE) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 89-96% | 89-96% | 80-89% | 97-105% |
EDTA plasma(n=5) | 83-97% | 96-103% | 85-94% | 84-91% |
heparin plasma(n=5) | 86-103% | 97-105% | 95-103% | 93-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:APOE) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Cerebrospinal fluid apolipoprotein E concentration decreases after seizureSeizure: PIIS1059131109002441
- Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant propertiesPubMed: 23328279
- Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinomaPubmed: 23609192
- Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human ObesityPubmed:25249572
- Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control studyPubMed: 25707516
- Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolismPubMed: 26515253
- Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesityPubMed: 25249572
- Adaptive immunity against gut microbiota enhances apoE-mediated immune regulation andreduces atherosclerosis and western-diet-related inflammation.pubmed:27383250
- Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD micePubmed:30026023
- Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse …Pubmed:29731975
- Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancyPubmed: 31162828
- Hepatic HuR modulates lipid homeostasis in response to high-fat dietPubmed: 32546794
- Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and CatsPubmed: 32880234
- The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome33321701
- Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer33602229
- GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity34853313
- Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled …Pubmed:35378356
- Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal …Pubmed:35361429