Multiplex Assay Kit for Brain Natriuretic Peptide (BNP) ,etc. by FLIA (Flow Luminescence Immunoassay) 
GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 449.00 US$ 467.00 US$ 492.00 US$ 527.00 US$ 562.00 US$ 613.00 US$ 691.00 US$ 864.00
- Quantity
Overview
Properties
- Product No.LMA541Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCardiovascular biologyNeuro scienceHormone metabolism
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Brain Natriuretic Peptide (BNP) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Brain Natriuretic Peptide (BNP) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-94 | 84 |
| EDTA plasma(n=5) | 97-105 | 102 |
| heparin plasma(n=5) | 85-103 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Brain Natriuretic Peptide (BNP) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-93% | 82-102% | 90-97% | 97-105% |
| EDTA plasma(n=5) | 88-95% | 94-102% | 93-101% | 80-103% |
| heparin plasma(n=5) | 96-104% | 79-91% | 83-93% | 89-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:BNP) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sicknessAPS: source
- Renalase deficiency in heart failure model of rats_a potential mechanism underlying circulating norepinephrine accumulation.PubMed: 21297953
- Cardiac Troponin T and NT-proBNP as Biomarkers of Early Myocardial Damage in Amitriptyline-Induced Cardiovascular Toxicity in RatsPubmed: 24055824
- ELEVATED ST2 SERUM LEVELS A BIOMARKER FOR CARDIOMYOPATHY IN DUCHENNE MUSCULAR DYSTROPHYOnlinejacc:Source
- Why does Adenotonsillectomy Not Correct Enuresis in All Children with Sleep Disordered Breathing?Pubmed:24679871
- Cardioprotective effects of baicalein on heart failure via modulation of Ca2PubMed: 26706290
- Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot studyPublication: 5950761
- Renal Sympathetic Denervation Improves Cardiac DysfunctionPubMed: 25804096
- Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitroPubmed:26706290
- Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathwayPubmed:26970569
- Improvement in the prediction of exercise-induced elevation of left ventricular filling pressure in patients with normal left ventricular ejection fraction.pubmed:27779336
- The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic …pubmed:27652141
- Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure.pubmed:27555054
- Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathypubmed:27693530
- Paracrine cross-talk between skeletal muscle and macrophages in exercise by PGC-1α-controlled BNP.pubmed:28091624
- Intermedin improves cardiac function and sympathetic neural remodeling in a rat model of post myocardial infarction heart failurepubmed:28627670
- Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathypubmed:29240788
- Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload pubmed:27575955
- Metformin attenuated endotoxin-induced acute myocarditis via activating AMPKpubmed:28410530
- Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy.pubmed:29063791
- Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity andPubmed:29727991
- An essential role for Wnt/β-catenin signaling in mediating hypertensive heart diseasePubmed:29895976
- Cardioprotective effect of substance P in a porcine model of acute myocardial infarctionPubmed:29884290
- High-intensity interval training enhances mitochondrial bioenergetics of platelets in patients with heart failurePubmed:30072155
- Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy SubjectsPubmed:29977313
- Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in ratsPubmed:29956781
- Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart DiseasePubmed:29392348
- Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosisPubmed:29321061
- 和肽素水平对大鼠心肾综合征的早期预测价值filename:ZBLS201802013
- Signature-oriented investigation of the efficacy of multicomponent drugs against heart failurePubmed: 30230922
- Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar ratsPubmed: 30426860
- Van Veterinary Journal
- Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndromePubmed: 30770217
- Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytesPubmed: 30982981
- Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart FailurePubmed: 31466059
- Pharmacodynamic and pharmacokinetic effects of S086, a novel angiotensin receptor neprilysin inhibitorPubmed: 32570118
- Empagliflozin improves left ventricular diastolic function of db/db micePubmed: 32353614
- Dapagliflozin: a Sodium-glucose Cotransporter 2 Inhibitor, Attenuates Angiotensin II-induced Cardiac Fibrotic Remodeling by Regulating TGF¦Â1/Smad ¡34116674
- Radiation-induced heart energy metabolism dysfunction leads to heart fibrosis
- κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a34373768
- Radiation‑induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues34633055
- Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model34452454
- Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stressPubmed:35218795