Multiplex Assay Kit for Calcitonin Gene Related Peptide (CGRP) ,etc. by FLIA (Flow Luminescence Immunoassay) 
CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 427.00 US$ 443.00 US$ 468.00 US$ 501.00 US$ 534.00 US$ 583.00 US$ 657.00 US$ 821.00
- Quantity
Overview
Properties
- Product No.LMA876Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityEndocrinologyNeuro scienceHormone metabolismBone metabolismDermatology
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Calcitonin Gene Related Peptide (CGRP) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Calcitonin Gene Related Peptide (CGRP) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-102 | 99 |
| EDTA plasma(n=5) | 85-92 | 88 |
| heparin plasma(n=5) | 85-99 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcitonin Gene Related Peptide (CGRP) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-99% | 90-97% | 90-97% | 79-89% |
| EDTA plasma(n=5) | 85-101% | 97-105% | 78-103% | 79-94% |
| heparin plasma(n=5) | 91-98% | 80-89% | 84-101% | 86-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CGRP) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo.Hindawi: 190370
- Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological markerPubMed: PMC3620076
- CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic MigraineOnlinelibrary: head.12372
- Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid APubmed:Pmc4028234
- Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot.Pubmed:24624298
- Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control studyPubmed:24943060
- OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migrainePubMed: 25735000
- Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in ratsPubMed: 25892548
- Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitisPubmed:27156748
- Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patientsPubmed:27250233
- Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction.Pubmed:27144592
- Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кBPubmed:27150105
- Prevention of Bone Loss in a Model of Postmenopausal Osteoporosis throughAdrenomedullin Inhibition.pubmed:27445864
- Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis.pubmed:27896372
- A new comorbidity model and the common pathological mechanisms of migraine and epilepsypubmed:28559979
- Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndromepubmed:28483500
- CNS myeloid cells critically regulate heat hyperalgesiaPubmed:29634489
- Feasibility of serum CGRP measurement as a biomarker of chronic migraine: a critical reappraisalPubmed:30006780
- RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis10.1101:357582
- MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL
- Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migrainePubmed: 31070784
- Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signalingPubmed: 31420791
- Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q-and T-type Ca 2+ currents in rat trigeminal ganglion …
- Effects of nanoparticles on Neuroinflammation in a Mouse Model of AsthmaPubmed: 31542455
- Electrical stimulation of the superior sagittal sinus suppresses A-type K currents and increases P/Q-and T-type Ca 2 currents in rat trigeminal ganglion …Pubmed: 31375062
- Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headachePubmed: 32286239
- Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practicePubmed: 32951099
- Mild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineurs33441852
- Perirenal adipose afferent nerves sustain pathological high blood pressure in ratsPubmed:35668093