Multiplex Assay Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7) ,etc. by FLIA (Flow Luminescence Immunoassay) 
CXCL7; PPBP; PBP; B-TG1; CTAP-III; CTAP3; CTAPIII; LA-PF4; LDGF; MDGF; NAP2; SCYB7; TC1; TC2; TGB1; THBGB1; Pro-Platelet Basic Protein; Beta-Thromboglobulin
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 427.00 US$ 443.00 US$ 468.00 US$ 501.00 US$ 534.00 US$ 583.00 US$ 657.00 US$ 821.00
- Quantity
Overview
Properties
- Product No.LMA370Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokineHematology
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Chemokine (C-X-C motif) ligand 7 ( CXCL7) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine (C-X-C motif) ligand 7 ( CXCL7) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-103 | 94 |
| EDTA plasma(n=5) | 78-99 | 87 |
| heparin plasma(n=5) | 97-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C motif) ligand 7 ( CXCL7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C motif) ligand 7 ( CXCL7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine (C-X-C motif) ligand 7 ( CXCL7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-102% | 94-103% | 83-97% | 85-99% |
| EDTA plasma(n=5) | 82-94% | 83-91% | 97-105% | 88-96% |
| heparin plasma(n=5) | 88-102% | 89-98% | 85-94% | 80-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#: CXCL7) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Chemokine (C-X-C motif) ligand 7 ( CXCL7) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Chemokine (C-X-C motif) ligand 7 ( CXCL7) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Chemokine (C-X-C motif) ligand 7 ( CXCL7) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Chemokine (C-X-C motif) ligand 7 ( CXCL7) in the sample and corresponding value of MFI (Median Fluorescence Intensity).
Giveaways
Increment services
Citations
- Strict heart rate control attenuates prothrombotic state and platelet activity in patients with non-valvular permanent atrial fibrillation.Pubmed: 2347822
- STUDIES ON THE STORAGE OF POOLED PLATELETS IN NON DOP PVC CONTAINERSEbscohost: Source
- Bioprofiling of platelets in medicated patients with depression.Pubmed:25451399
- Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testingPubMed: 26243277
- Differential changes in platelet reactivity induced by acute physical compared to persistent mental stressPubMed: 26192713
- Bioprofiling of platelets in medicated patients with depressionPubMed: 25451399
- Markers of coagulation and fibrinolysis do not detect or predict the presence of left atrial appendage thrombus in patients with atrial fibrillationPubmed: 32147650
- A novel di‐peptide Met‐Glu from collagen hydrolysates inhibits platelet aggregation and thrombus formation via regulation of Gq‐mediated signalingPubmed: 32662128
- Endogenous Nitric Oxide-Releasing Microgel Coating Prevents Clot Formation on Oxygenator Fibers Exposed to In Vitro Blood FlowPubmed:35054599
- The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female ratsPubmed:35183388