Multiplex Assay Kit for Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) 
CD26; ADA; DPPIV; DPP-IV; DPP4; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26; Dipeptidyl Peptidase IV
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 393.00 US$ 408.00 US$ 431.00 US$ 461.00 US$ 491.00 US$ 537.00 US$ 605.00 US$ 756.00
- Quantity
Overview
Properties
- Product No.LMA884Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionEnzyme & KinaseApoptosisTumor immunityEndocrinologyCardiovascular biology
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 85-101 | 91 |
| EDTA plasma(n=5) | 94-105 | 101 |
| heparin plasma(n=5) | 84-91 | 88 |
| sodium citrate plasma(n=5) | 95-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-97% | 85-92% | 80-95% | 87-94% |
| EDTA plasma(n=5) | 80-101% | 78-90% | 80-103% | 87-98% |
| heparin plasma(n=5) | 86-101% | 88-96% | 87-94% | 83-90% |
| sodium citrate plasma(n=5) | 97-105% | 78-93% | 88-95% | 90-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CD26) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
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Citations
- Linagliptin Blocks Renal Damage in Type 1 Diabetic Rats by Suppressing Advanced Glycation End Products-Receptor AxisPubmed: 24710699
- MK-0626, a dipeptidyl peptidase-4 inhibitor, improves neovascularization by increasing both the number of circulating endothelial progenitor cells and endothelial nitric oxide synthetase expression.Pubmed:24059225
- Ghrelin inhibition restores glucose homeostasis in hepatocyte nuclear factor-1alpha (MODY3) deficient micePubMed: 25979074
- Expression and Clinical Significance of Serum Dipeptidyl Peptidase IV Chronic Obstructive Pulmonary DiseasePubmed:26992252
- Expression of recombinant human α-lactalbumin in milk of transgenic cloned pigs is sufficient to enhance intestinal growth and weight gain of suckling pigletsPubmed:26899869
- Hepatic Spheroids for Long-Term Toxicity Studiesaivt.2016.0016
- High circulating plasma dipeptidyl peptidase- 4 levels in non-obese Asian Indians with type 2 diabetes correlate with fasting insulin and LDL-C levels, triceps skinfolds, total intra-abdominal adipose tissue volume and presence of diabetes: a case–control study10.1136:bmjdrc-2017-000393
- Dipeptidyl peptidase-4 levels are increased and partially related to body fat distribution in patients with familial partial lipodystrophy type 2pubmed:28450900
- The effect of CD26‐deficiency on dipeptidyl peptidase 8 and 9 expression profiles in a mouse model of Crohn's diseasePubmed:29693275
- Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activityPubmed:29953010
- The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairmentPubmed:35468904