Multiplex Assay Kit for Connective Tissue Growth Factor (CTGF) ,etc. by FLIA (Flow Luminescence Immunoassay) 
CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 472.00 US$ 490.00 US$ 517.00 US$ 553.00 US$ 590.00 US$ 644.00 US$ 726.00 US$ 907.00
- Quantity
Overview
Properties
- Product No.LMA010Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokine
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Connective Tissue Growth Factor (CTGF) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Connective Tissue Growth Factor (CTGF) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-94 | 87 |
| EDTA plasma(n=5) | 93-102 | 99 |
| heparin plasma(n=5) | 97-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Connective Tissue Growth Factor (CTGF) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-105% | 81-97% | 80-98% | 84-101% |
| EDTA plasma(n=5) | 88-97% | 94-103% | 81-89% | 82-104% |
| heparin plasma(n=5) | 80-92% | 98-105% | 94-101% | 79-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CTGF) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis modelPubMed: 19824055
- Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell functionPubMed: PMC2726461
- Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound HealingPlosone: 0032128
- Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathyScienceDirect: S0009912012000987
- Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development?PubMed: S1053249812010121
- The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS RatsBesjournal: Source
- Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosisPubMed: 22846210
- Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosisPubMed: PMC3351780
- Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats.Pubmed: 24211397
- Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapyRo-journal: Source
- Knock Out of S1P3 Receptor Signaling Attenuates Inflammation and Fibrosis in Bleomycin-Induced Lung Injury Mice ModelPubmed:Pmc4157792
- Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal modelsPubmed:24464932
- Connective Tissue Growth Factor is a Target of Notch Signaling in Cells of the Osteoblastic LineagePubmed:24792956
- Disruption of collagen homeostasis can reverse established age-related myocardial fibrosisPubmed:25701883
- Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitisPubMed: 26190501
- Suppression of connective tissue growth factor mediates the renoprotective effect of Sitagliptin rather than Pioglitazone in type 2 diabetes mellitusPubmed:27049870
- TGF-β and Physiological Root Resorption of Deciduous Teeth.pubmed:28035998
- Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathypubmed:27516211
- Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRRpubmed:28004175
- The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidencepubmed:27531356
- Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseasespubmed:27686729
- Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathwaypubmed:28698735
- Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR10.1007/s00380-016-0930-y
- 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis.pubmed:28460256
- Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events.pubmed:29120858
- Sustained β-AR stimulation induces synthesis and secretion of growth factors in cardiac myocytes that affect on cardiac fibroblast activationPubmed:29107793
- Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller CellsPubmed:30123889
- TGFβ mediates collagen production in human CRSsNP nasal mucosa‐derived fibroblasts through Smad2/3‐dependent pathway and CTGF induction and secretionPubmed: 30426494
- Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with …Pubmed: 30413105
- Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin MPubmed: 31249305
- Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart FailurePubmed: 31466059
- CTGF Attenuates Tendon-Derived Stem/Progenitor Cell AgingPubmed: 31827530
- Small-Dose Sunitinib Modulates p53, Bcl-2, STAT3, and ERK1/2 Pathways and Protects against Adenine-Induced NephrotoxicityPubmed: 33212804
- Lysophosphatidic acid as a regulator of endometrial connective tissue growth factor and prostaglandin secretion during estrous cycle and endometrosis in the …Pubmed: 32943074
- MicroRNAs and ventricular remodeling in aortic stenosisPubmed: 32682570
- Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction
- High carbohydrate high fat diet causes arterial hypertension and histological changes in the aortic wall in aged rats: The involvement of connective tissue growth …34455071
- Hepatocyte-specific Smad4 deficiency alleviates liver fibrosis via the p38/p65 pathway