Multiplex Assay Kit for Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) Oryctolagus cuniculus (Rabbit) Multiplex ELISA

Hydrocortisone

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA462Rb.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-103 90
EDTA plasma(n=5) 79-95 82
heparin plasma(n=5) 80-103 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-101% 95-103% 80-97% 80-89%
EDTA plasma(n=5) 94-102% 97-105% 80-96% 80-105%
heparin plasma(n=5) 79-94% 87-95% 91-98% 86-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:Cor) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.

Giveaways

Increment services

Citations

  • Use of infrared thermography to assess the influence of high environmental temperature on rabbitsScienceDirect: S0034528813001380
  • Identification of sociodemographic and clinical factors associated with the levels of human b-defensin-1 and human b-defensin-2 in the human milk of Han ChinesePubmed:24124699
  • Daily exposure to summer circadian cycles affects spermatogenesis, but not fertility in an in vivo rabbit modelPubmed:25442388
  • Effect of high temperatures on breeding rabbit behaviourPublish:Source
  • Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental studentsIndex.Php: Jbcd
  • Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in ratsPubMed: 26684638
  • Surface plasmon resonance immunoassay for cortisol determination with a self-assembling denaturalised bovine serum albumin layer on surface plasmon resonance chipxpls:7393900
  • Fabrication and characterization of SPR chips with the modified bovine serum albuminarticle:10.1007
  • Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calvespublication:306322503
  • Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactationacve-2017-0006
  • Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine …Pubmed:29540178
  • Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition:
  • Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation …
  • Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons
  • A study on certain blood biomarkers of pregnant Cholistani cows.
  • The Use of Infrared Thermography (IRT) as Stress Indicator in Horses Trained for Endurance: A Pilot StudyPubmed: 30866503
  • Saliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexiaPubmed: 32028434
  • Assessment of certain biomarkers for predicting survival in response to treatment in dogs naturally infected with canine parvovirusPubmed: 32926999
  • Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition
  • Physiological responses to salinity stress in the Managua Cichlid, Cichlasoma managuense
  • Anti-stress activity of some plants extracts of the North Caucasus flora
  • Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves33610512
  • Exploring alterations in hematological and biochemical parameters, enzyme activities and serum cortisol in Besnoitia besnoiti naturally infected dairy cattle33722267
  • Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest33960200
  • The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets33435211
  • Stress response after race and endurance training sessions and competitions in Arabian horses33497894
  • Associations of Chinese social face with cortisol level and glucocorticoid receptor gene
  • Orexin-A/OX1 Receptor Regulates Autophagy, Mitochondrial Autophagy, and Cortisol Secretion in Human Adrenocortical Cells Through the mTORC1/P70s6k1 …

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