Multiplex Assay Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay) Oryctolagus cuniculus (Rabbit) Multiplex ELISA

CTX-I; CTX1;

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA665Rb.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-91 85
EDTA plasma(n=5) 90-101 93
heparin plasma(n=5) 88-99 93
sodium citrate plasma(n=5) 87-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-101% 85-102% 92-99% 90-99%
EDTA plasma(n=5) 82-95% 96-104% 78-95% 98-105%
heparin plasma(n=5) 83-102% 92-101% 78-96% 86-95%
sodium citrate plasma(n=5) 94-102% 82-103% 86-104% 79-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CTXI) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.

Giveaways

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Citations

  • Atp6v0d2 Is an Essential Component of the Osteoclast-Specific Proton Pump That Mediates Extracellular Acidification in Bone ResorptionPubMed: PMC2672205
  • Borage and fish oils lifelong supplementation decreases inflammation and improves bone health in a murine model of senile osteoporosisPubMed: 21664309
  • Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial.Trials: 1745-6215
  • TRAF family member-associated NF-κB activator (TANK) is a negative regulator of osteoclastogenesis and bone formationPubMed: 22773835
  • A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular RiskPubMed: PMC3462931
  • Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat GainEndocrine: Source
  • DPP IV inhibitor treatment attenuates bone loss and improves mechanical bone strength in male diabetic ratsPubmed:25053403
  • Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry diseasePubmed:25073565
  • Bone resorption correlates with the frequency of CD5+ B cells in the blood of patients with rheumatoid arthritisPubmed:25193807
  • XPO1/CRM1-Selective Inhibitors of Nuclear Export (SINE) reduce tumor spreading and improve overall survival in preclinical models of prostate cancer (PCa)Pubmed:25284315
  • The presence of FCGR2B promoter or transmembrane region variant alleles leads to reduced serum IL-6 levels in rheumatoid arthritisPubmed:25630523
  • 5-azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survivalPubmed:25691576
  • Osteocartilaginous metabolic markers change over a 3-week stage race in pro-cyclistsPubMed: 26174975
  • Effects of renal tubular dysfunction on bone in tenofovir-exposed HIV-positive patientsPubMed: 26372384
  • Biomarkers in Remission According to Different Criteria in Patients with Rheumatoid ArthritisPubMed: 26472417
  • Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathwayPubMed: 26677102
  • Mutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast FunctionPubMed: 25837253
  • Oxytocin reverses osteoporosis in a sex-dependent mannerPubMed: 26042090
  • The RIP1–RIP3 Complex Mediates Osteocyte Necroptosis after Ovariectomy in Ratspmc:PMC4795547
  • Lanthanum Chloride Attenuates Osteoclast Formation and Function Via the DownreCavia (Guinea pig )lation of Rankl-Induced Nf-κb and Nfatc1 ActivitiesPubmed:26060084
  • N-Homocysteinylation impairs collagen cross-linking in cystathionine β-synthase-deficient mice: a novel mechanism of connective tissue abnormalities.pubmed:27530978
  • High FGF21 levels are associated with altered bone homeostasis in HIV-1-infected patients.pubmed:28521869
  • The effect of calcium and vitamin D supplementation on bone health of male Jockeys.pubmed:27568072
  • Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathwaypubmed:27387537
  • The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar ratspubmed:27838501
  • Reduced femoral bone mass in both diet-induced and genetic hyperlipidemia micepubmed:27669658
  • A potential role for glycated cross-links in abdominal aortic aneurysm disease.pubmed:27624703
  • Effects of long-term administration of pantoprazole on bone mineral density in young male rats.pubmed:27552060
  • Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized ratsS0753332216322752
  • Cannabidiol administration reduces sublesional cancellous bone loss in rats with severe spinal cord injurypubmed:28479140
  • Side-Effects of Convulsive Seizures and Anti-Seizure Therapy on Bone in a Rat Model of Epilepsypubmed:28905646
  • Obesity status influences the relationship among serum osteocalcin, iron stores and insulin sensitivitypubmed:29050649
  • A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice.pubmed:28060768
  • 骨形成指标PINP 与骨吸收指标β-CTX 在2 型糖尿病合并骨质疏松中作用的研究.10.3969/j.issn.1006-7108.2017.03.009
  • Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Researchpubmed:29119115
  • TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42Pubmed:29358671
  • The role of androgens on periodontal repair in female ratsPubmed:29683499
  • Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathwayPubmed:29883721
  • The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolismPubmed:29428468
  • Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX studyPubmed:30076009
  • Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive RatsPubmed:29898457
  • Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesisPubmed:29805533
  • Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice10.3892:etm.2018.6353
  • Dual PI3 K/mTOR inhibition reduces prostate cancer bone engraftment altering tumor-induced bone remodelingPubmed:29687745
  • Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related BiomarkersPubmed: 30181433
  • Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway
  • Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise
  • Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed …Pubmed: 30983556
  • Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumorsPubmed: 30952517
  • Significance of the Tks4 scaffold protein in bone tissue homeostasis
  • E The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats
  • Osteocyte TSC1 promotes sclerostin secretion to restrain osteogenesis in micePubmed: 31088250
  • Role of testosterone and androgen receptor in periodontal disease progression in female ratsPubmed: 31389012
  • Biomarkers of oral inflammation in perinatally HIV‐infected and perinatally HIV exposed, uninfected youthPubmed: 31385616
  • Laboratory Monitoring of Bone Tissue Remodeling after Augmentation of Impression Intraarticular Fracture with Different Types of Bone GraftPubmed: 31630302
  • AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activitiesPubmed: 31739209
  • Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar RatsPubmed: 31739465
  • Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic PatientsPubmed: 31717649
  • Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signalsPubmed: 31826180
  • BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodelingPubmed: 31914211
  • EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for OsteoporosisPubmed: 32194868
  • Article Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous …Pubmed: 31877673
  • RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin SynthesisPubmed: 32640190
  • A Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype …Pubmed: 32670749
  • The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats.
  • Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous …
  • Qu Feng Zhi Tong capsule increases mechanical properties of cortical bone in ovariectomized rats
  • 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice
  • The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats
  • 淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究
  • Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza bağlı kırık riskinin ve kemik Turnoveri'nin değerlendirilmesi
  • A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair33310848
  • Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway33950533
  • Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis34233019
  • Effects of icariin on the fracture healing in young and old rats and its mechanism34511043
  • Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway34512780
  • Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy34296634
  • Night shift work and osteoporosis-bone turnover markers among female blue-collar workers in PolandPubmed:35139709
  • Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progressionPubmed:35235775
  • BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axisPubmed:35523787
  • Foxf2 represses bone formation via Wnt2b/β-catenin signalingPubmed:35668101
  • Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticlesPubmed:35386445
  • Puerarin specifically disrupts osteoclast activation via blocking integrin-β3 Pyk2/Src/Cbl signaling pathwayPubmed:35228997
  • Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35 …

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