Multiplex Assay Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay) Mus musculus (Mouse) Multiplex ELISA

FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA559Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-104 96
EDTA plasma(n=5) 92-103 95
heparin plasma(n=5) 83-96 90
sodium citrate plasma(n=5) 93-104 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 85-99% 95-102% 80-101%
EDTA plasma(n=5) 92-99% 96-105% 80-99% 84-98%
heparin plasma(n=5) 79-91% 78-101% 80-94% 78-97%
sodium citrate plasma(n=5) 87-103% 91-98% 83-94% 83-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FABP2) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytesPubMed: 25952883
  • Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation ModelPubMed: 26064429
  • Immunomodulatory potential of β-glucan as supportive treatment in porcine rotavirus enteritis.pubmed:28895864
  • Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed ratspubmed:28622357
  • Effect of Porcine Rotavirus Enteritis on Concentrations of Interferonγ, Immunoglobulin-G and Intestinal Fatty Acid-Binding Protein-2 and Peripheral Leukocyte Function10.5958/0973-9149.2017.00011.9
  • Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory …Pubmed:29672286
  • Lack of liver steatosis in germ-free mice following hypercaloric dietsPubmed:29926176
  • Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and IronPubmed:29861488
  • Intestinal fatty acid binding protein (I-FABP) as a marker for acute intestinal ischemiaDoi: 10.18203/2349-2902.isj20190058
  • Effect of a Multistrain Probiotic on Cognitive Function and Risk of Falls in Patients With Cirrhosis: A Randomized Trial
  • Immunomodulating dose of levamisole stimulates innate immune response and prevents intestinal damage in porcine rotavirus diarrhea: a restricted-randomized …Pubmed: 30790158
  • Role of Aquaporin-3 in Intestinal Injury Induced by SepsisPubmed: 31582652
  • FABP1 and FABP2 as markers of diabetic nephropathyPubmed: 32922200
  • Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory responsePubmed: 32361189
  • Markers of Intestinal Damage in Individuals with and without Obesity during a 12-Week Exercise Period.
  • Effects of fucoidan on tumor prevention and gut flora
  • Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury33514869
  • The ¦Á2AR/Caveolin©\1/p38MAPK/NF©\¦ÊB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia©\reperfusion34114328
  • Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity34022802
  • Impact of Successive Exertional Heat Injuries on Thermoregulatory and Systemic Inflammatory Responses in Mice34528459
  • The protective effects of HIF-1α activation on sepsis induced intestinal mucosal barrier injury in rats model of sepsisPubmed:35576220

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