Multiplex Assay Kit for Fatty Acid Binding Protein 5 (FABP5) ,etc. by FLIA (Flow Luminescence Immunoassay)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 5 (FABP5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 5 (FABP5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Anti-Fatty Acid Binding Protein 5 (FABP5) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Fatty Acid Binding Protein 5 (FABP5) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Fatty Acid Binding Protein 5 (FABP5) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Fatty Acid Binding Protein 5 (FABP5) in the sample and corresponding value of MFI (Median Fluorescence Intensity).