Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) Rattus norvegicus (Rat) Multiplex ELISA

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA518Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-101 97
EDTA plasma(n=5) 97-105 102
heparin plasma(n=5) 78-101 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 80-102% 87-105% 88-98%
EDTA plasma(n=5) 89-98% 95-104% 78-105% 96-103%
heparin plasma(n=5) 93-102% 91-98% 87-97% 84-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FE) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

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Citations

  • PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation.Pubmed: 23438894
  • Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma.PubMed: 25804229
  • Host H68D Genotype Affects Tumor Growth in Mouse MelanomaOpen-Access: 1948-5956-1000353
  • The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing micePubmed:26757699
  • The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Diseasepubmed:27782743
  • 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain.pubmed:27615544
  • Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activationpubmed:29147463
  • sucrosomial® iron absorption studied by in vitro and ex-vivo modelspubmed:29055735
  • In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells.pubmed:29051659
  • Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women10.1590/MEDICC.2017.1902030004 
  • The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus.pubmed:28584599
  • Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system10.1016/j.fbio.2017.10.001
  • Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Ratspubmed:28629118
  • Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cellsPubmed:29622185
  • Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet10.1016:j.anifeedsci.2018.01.012
  • Iron homeostasis in pregnancy and spontaneous abortionPubmed: 30394565
  • Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria
  • New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemiaPubmed: 30792208
  • PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE
  • Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic
  • Transferrin plays a central role in coagulation balance by interacting with clotting factorsPubmed: 31811276
  • Gum arabic capped copper nanoparticles: Synthesis, characterization, and applicationsPubmed: 31904465
  • Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State FermentationPubmed: 32255407
  • Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability
  • Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure34139233
  • The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11Pubmed:35700835

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