Multiplex Assay Kit for Gamma-Aminobutyric Acid (gABA) ,etc. by FLIA (Flow Luminescence Immunoassay) 
4-Aminobutyric Acid
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 420.00 US$ 436.00 US$ 461.00 US$ 493.00 US$ 525.00 US$ 574.00 US$ 646.00 US$ 808.00
- Quantity
Overview
Properties
- Product No.LMA900Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionCardiovascular biologyNeuro scienceBone metabolism
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Gamma-Aminobutyric Acid (gABA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Gamma-Aminobutyric Acid (gABA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 82-101 | 97 |
| heparin plasma(n=5) | 86-101 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gamma-Aminobutyric Acid (gABA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-104% | 95-105% | 86-96% | 89-98% |
| EDTA plasma(n=5) | 96-105% | 91-98% | 80-98% | 83-98% |
| heparin plasma(n=5) | 94-105% | 78-101% | 80-91% | 87-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:gABA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellumPubMed: 21444355
- Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear.PubMed: 18577424
- Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with AutismPubMed: PMC3623607
- Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic ChildrenPubMed: PMC3694825
- Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)PubMed: PMC3762604
- Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertensionpubmed:27917928
- Sex differences in the glutamate signaling pathway in juvenile ratsPubmed:28861894
- A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzymePubmed: 30341396
- Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of NeurotransmissionPubmed: 30666488
- Differentiation of human glioblastoma U87 cells into cholinergic neuronPubmed: 30928478
- Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism
- Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.
- Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsyPubmed: 33101467
- Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation33828024
- Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases34101060
- Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission34475508
- Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studiesPubmed:35102569