Multiplex Assay Kit for Glucose 6 Phosphate Isomerase (GPI) ,etc. by FLIA (Flow Luminescence Immunoassay) 
AMF; NLK; PHI; SA36; Phosphoglucose Isomerase; Autocrine motility factor; Neuroleukin; Phosphohexose isomerase; Sperm antigen 36
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 427.00 US$ 443.00 US$ 468.00 US$ 501.00 US$ 534.00 US$ 583.00 US$ 657.00 US$ 821.00
- Quantity
Overview
Properties
- Product No.LMA725Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathway
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Glucose 6 Phosphate Isomerase (GPI) and the recovery rates were calculated by comparing the measured value to the expected amount of Glucose 6 Phosphate Isomerase (GPI) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-105 | 99 |
| EDTA plasma(n=5) | 84-98 | 89 |
| heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose 6 Phosphate Isomerase (GPI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose 6 Phosphate Isomerase (GPI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glucose 6 Phosphate Isomerase (GPI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-102% | 90-97% | 87-101% | 79-96% |
| EDTA plasma(n=5) | 95-105% | 80-103% | 87-97% | 90-97% |
| heparin plasma(n=5) | 98-105% | 80-99% | 87-96% | 82-89% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:GPI) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Glucose 6 Phosphate Isomerase (GPI) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Glucose 6 Phosphate Isomerase (GPI) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Glucose 6 Phosphate Isomerase (GPI) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Glucose 6 Phosphate Isomerase (GPI) in the sample and corresponding value of MFI (Median Fluorescence Intensity).
Giveaways
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Citations
- Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factorPubMed: 23027359
- Bruton's tyrosine kinase deficiency inhibits autoimmune arthritis but fails to block immune complex‐mediated inflammatory arthritisPubmed:26945549
- Bruton's Tyrosine Kinase Deficiency Inhibits Autoimmune Arthritis in Mice but Fails to Block Immune Complex–Mediated Inflammatory Arthritispubmed:26945549
- The GPI transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malariaPubmed:29784863
- Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motilityPubmed: 31394311
- The Asthma-associated PER1-like domain-containing protein 1 (PERLD1) Haplotype Influences Soluble Glycosylphosphatidylinositol Anchor Protein (sGPI …Pubmed: 31959860
- T–B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated ProteinPubmed: 33199538