Multiplex Assay Kit for Glutathione Peroxidase 1 (GPX1) ,etc. by FLIA (Flow Luminescence Immunoassay) 
GSHPX1; Cellular glutathione peroxidase
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 405.00 US$ 420.00 US$ 443.00 US$ 475.00 US$ 506.00 US$ 552.00 US$ 622.00 US$ 778.00
- Quantity
Overview
Properties
- Product No.LMA295Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseMetabolic pathway
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Glutathione Peroxidase 1 (GPX1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Glutathione Peroxidase 1 (GPX1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-99 | 91 |
| EDTA plasma(n=5) | 83-99 | 94 |
| heparin plasma(n=5) | 80-101 | 82 |
| sodium citrate plasma(n=5) | 83-93 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glutathione Peroxidase 1 (GPX1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-102% | 81-90% | 87-95% | 94-104% |
| EDTA plasma(n=5) | 91-105% | 90-98% | 90-104% | 80-90% |
| heparin plasma(n=5) | 92-101% | 89-103% | 79-105% | 93-104% |
| sodium citrate plasma(n=5) | 85-96% | 85-99% | 81-99% | 80-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:GPX1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine SupplementationPlos:Source
- Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closurePubmed:24443098
- Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal CancerPubmed:25550558
- Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, PubMed: 26674569
- Seasonal Variation in Glutathione Peroxidase in Seminal Plasma of Karan Fries (Tharparkar X Holstein Friesian) Bulls Under Tropical Climatic Conditionspublication:301246301
- Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditionsfileID:697741
- Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanismpubmed:27599793
- Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas.pubmed:28574428
- Assessing the serum concentration levels of NT-4/5, GPX-1, TNF-α, and l-arginine as biomediators of depression severity in first depressive episode patients with and without posttraumatic stress disorder.pubmed:28958613
- Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot studypubmed:29248027
- Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stresspubmed:28654832
- Selenium- Essential Antioxidant Elemen the example of autoimune thyroiditispdf:44%20PREDA%20C%207%2017.pdf
- Seasonal variation of mitochondria activity related and heat shock protein genes in spermatozoa of Karan Fries bulls in tropical climate10.1080:09291016.2017.1361584
- Investigation of GPX1 Gene Expression, Serum GPX1 and Selenium Levels on Colorectal Cancer
- Kolon Kanserinde Gpx1 Gen Anlatımı, Serum Gpx1 ve Selenyum Düzeylerinin İncelenmesi
- Influence of deficient nutrition on trace element status and antioxidant defense systemPubmed: 1781925
- Glutathione, glutathione peroxidase and some hematological parameters of HIV-seropositive subjects attending clinic in University of Calabar teaching …Pubmed: 31703562
- Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathwayPubmed: 32798557
- AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS
- In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells
- Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡33689849
- Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis34945751
- Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver diseasePubmed:35086715
- A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating …Pubmed:35281603