Multiplex Assay Kit for Hyaluronic Acid (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) 
Hyaluronan; Hyaluronate
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 497.00 US$ 516.00 US$ 544.00 US$ 583.00 US$ 621.00 US$ 678.00 US$ 764.00 US$ 955.00
- Quantity
Overview
Properties
- Product No.LMA182Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityHepatology
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Hyaluronic Acid (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Hyaluronic Acid (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-95 | 92 |
| EDTA plasma(n=5) | 78-104 | 90 |
| heparin plasma(n=5) | 80-103 | 83 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronic Acid (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronic Acid (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hyaluronic Acid (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-90% | 87-96% | 97-104% | 83-102% |
| EDTA plasma(n=5) | 89-97% | 88-96% | 86-101% | 78-101% |
| heparin plasma(n=5) | 94-102% | 90-97% | 89-101% | 88-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:HA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Hemorrhagic Fever Caused by a Novel Bunyavirus in China: Pathogenesis and Correlates of Fatal OutcomeOxfordJournals: source
- Alveolar fluid in acute respiratory distress syndrome promotes fibroblast migration: Role of platelet-derived growth factor pathway*PubMed: 22713216
- Age effects on extracellular matrix production of vocal fold scar fibroblasts in ratsPubmed: 24077847
- Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro StudyScienceDirect: S0892199713002415
- Resveratrol inhibits ovarian cancer cell adhesion to peritoneal mesothelium in vitro by modulating the production of α5β1 integrins and hyaluronic acidPubmed:24995580
- Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propriaPubmed:25545625
- Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosisPubMed: 25936962
- Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-5 Infected Patients in EgyptPubMed: 26492131
- Study of Some Serum Biochemical Markers of Liver Fibrosis in Patients with Chronic HCVISSN 2575-7733
- A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritispubmed:29166937
- Polycomb group proteins: Novel molecules associated with ultraviolet A-induced photoaging of human skin.pubmed:28962194
- The effect of aerobic walking and lower body resistance exercise on serum COMP and hyaluronan, in both males and femalesPubmed:29536174
- Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis10.1007:s00217-018-3119-z
- Juvenile Ovine Ex Vivo Larynges: Phonatory, Histologic, and Micro CT Based Anatomic Analyses
- Selected elements of extracellular matrix of the skin in diabetes and insulin resistancePubmed: 31146169
- Strain-Dependent Porcine Circovirus Type 2 (PCV2) Entry and Replication in T-LymphoblastsPubmed: 31480752
- Betulin attenuated liver damage by prevention of hepatic mitochondrial dysfunction in rats with alcoholic steatohepatitisPubmed: 31619044
- Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortPubmed: 32210109
- iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma33540066
- An exploratory study to investigate the association between age, physical activity, femoral trochlear cartilage thickness and biomarkers of tissue metabolism in?¡33713200
- Trimethylamine N‐oxide exacerbates acetaminophen‐induced liver injury by interfering with macrophage‐mediated liver regeneration34459512
- Comparative studies on the effect of pig adipose-derived stem cells (pASCs) preconditioned with hypoxia or normoxia on skin wound healing in micePubmed:35718003
- Bicyclol ameliorates advanced liver diseases in murine models via inhibiting the IL-6/STAT3 signaling pathwayPubmed:35658240
- Novel multifunctional delivery system for chondrocytes and articular cartilage based on carbon quantum dots