Multiplex Assay Kit for Indole 3 Acetic Acid (IAA) ,etc. by FLIA (Flow Luminescence Immunoassay)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Anti-Indole 3 Acetic Acid (IAA) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Indole 3 Acetic Acid (IAA) and unlabeled Indole 3 Acetic Acid (IAA) (Standards or samples) with the pre-coated antibody specific to Indole 3 Acetic Acid (IAA). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Indole 3 Acetic Acid (IAA) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Indole 3 Acetic Acid (IAA) in the sample.