Multiplex Assay Kit for Interleukin 12 (IL12) ,etc. by FLIA (Flow Luminescence Immunoassay) 
p70
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 348.00 US$ 362.00 US$ 382.00 US$ 409.00 US$ 436.00 US$ 476.00 US$ 536.00 US$ 670.00
- Quantity
Overview
Properties
- Product No.LMA111Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- Category
Sign into your account
Share a new citation as an author
Upload your experimental result
Review
Contact us
Please fill in the blank.
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 12 (IL12) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 12 (IL12) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 87-103 | 95 |
| EDTA plasma(n=5) | 90-104 | 96 |
| heparin plasma(n=5) | 80-101 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12 (IL12) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12 (IL12) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 12 (IL12) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-104% | 95-102% | 96-105% | 87-94% |
| EDTA plasma(n=5) | 85-99% | 98-105% | 92-101% | 91-105% |
| heparin plasma(n=5) | 85-96% | 98-105% | 80-104% | 94-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:IL12) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.pubmed:
- A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme …Scholarena:Source
- Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experimentPubmed:30015235
- Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitroPubmed:30127867
- The P2X7 receptor, cathepsin S and fractalkine in the trigeminal subnucleus caudalis signal persistent hypernociception in temporomandibular rat jointsPubmed: 30248434
- Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in …Pubmed: 30223387
- The relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis mice
- ВЛИЯНИЕ ДНК МЕТИЛТРАНСФЕРАЗЫ 3А И ГИСТОНДЕАЦЕТИЛАЗЫ НА СОДЕРЖАНИЕ В МОНОНУКЛЕАРНЫХ КЛЕТКАХ ЦЕЛЬНОЙ КРОВИ …
- RBP and Alb in neonatal hydronephrosis and its association with IL-12 levels in pregnant women before delivery
- Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus
- Disseminated And Infection Accompanied Sweet's Syndrome In A Patient With Anti-Interferon-γ Autoantibodies: A Case Report.Pubmed: 31632104
- Suppression of Fpr2 expression protects against endotoxin-induced acute lung injury by interacting with Nrf2-regulated TAK1 activationPubmed: 32106380
- Silkworm pupa oil attenuates acetaminophen‐induced acute liver injury by inhibiting oxidative stress‐mediated NF‐κB signalingPubmed: 31993149
- Innate immune response of mammary gland induced by intramammary infusion of Bifidobacterium breve in lactating dairy cowsPubmed: 33071254
- Vasküler Demans Modelinde Çevresel Zenginleştirmenin Depresyon Benzeri Davranış, Kortikal ve Hipokampal BDNF ve IL-1β Seviyeleri Üzerine Etkisi
- Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra
- BBG enhances OLT1177-induced NLRP3 inflammasome inactivation by targeting P2X7R/NLRP3 and MyD88/NF-¦ÊB signaling in DSS-induced colitis in rats33548287
- HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED134117711
- Establishing novel roles of bifidocin LHA, antibacterial, antibiofilm and immunomodulator against Pseudomonas aeruginosa corneal infection model34171261
- Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma34377237
- Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study34730850
- Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes34829910
- Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBMPubmed:35351131
- Cyclosporine A loaded brain targeting nanoparticle to treat cerebral ischemia/reperfusion injury in micePubmed:35658867
- Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine ModelPubmed:35740356