Multiplex Assay Kit for Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) Homo sapiens (Human) Multiplex ELISA

HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA785Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-101 97
EDTA plasma(n=5) 91-101 95
heparin plasma(n=5) 85-99 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-101% 80-95% 83-101% 80-103%
EDTA plasma(n=5) 91-99% 88-104% 86-101% 87-103%
heparin plasma(n=5) 89-98% 81-99% 92-99% 91-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:Kim1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxicationPubMed: 19827907
  • KIM-1 and NGAL: new markers of obstructive nephropathySpringerLink: v461592787w5488x
  • Urinary Neutrophil Gelatinase-Associated Lipocalin at Birth Predicts Early Renal Function in Very Low Birth Weight InfantsLWW: 10000
  • Markers of kidney function in the elderly in relation to the new CKD-EPI formula for estimation of glomerular filtration rateAMS: source
  • Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product contentPubMed: 23046363
  • Comprehensive assessment of nephrotoxicity of intravenously administered sodium-oleate-coated ultra-small superparamagnetic iron oxide (USPIO) and titanium dioxide (TiO(2)) nanoparticles in rats.PubMed: 23272807
  • A Mouse Diversity Panel Approach Reveals the Potential for Clinical Kidney Injury Due to DB289 Not Predicted by Classical Rodent ModelsPubMed: PMC3498743
  • Interleukin 18 as a Marker of Chronic Nephropathy in Children after Anticancer TreatmentHindawi: 369784
  • Urinary Measurement of Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Helps Diagnose Acute Pyelonephritis in a Preclinical ModelHindawi: 413853
  • Kidney injury molecule-1: a urinary biomarker for contrast induced acute kidney injury.Iiste: Source
  • Urinary neutrophil gelatinase-associated lipocalin predicts graft loss after acute kidney injury in kidney transplantPubmed: 24325180
  • Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content.Pubmed: 23046363
  • The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary StudiesHindawi: 740105
  • Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat modelPubmed:25476829
  • Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty ratsSagepub:Source
  • 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义Jcp-Sh:Source
  • Tubular urinary biomarkers do not identify aetiology of acute kidney injury in kidney transplant recipientsPubmed:24666430
  • Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-ReperfusionPubmed:24816434
  • SYNbiotics Easing Renal failure by improving Gut microbiologY (SYNERGY): a protocol of placebo-controlled randomised cross-over trialPubmed:24996842
  • The diagnosis of febrile urinary tract infection in children may be facilitated by urinary biomarkersPubmed:25127917
  • Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous CrisisPubmed:25448261
  • Kidney injury molecule-1: A urinary biomarker for contrast-induced acute kidney injuryTmjournals:Source
  • Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitisPubMed: 26191125
  • Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in micePubMed: 26045773
  • Vorinostat protects against calcium oxalate-induced kidney injury in micePubMed: 26095064
  • Role of new biomarkers for predicting renal scarring in vesicoureteral reflux: NGAL, KIM-1, and L-FABPPubMed: 26324091
  • Urinary NGAL, KIM-1 and L-FABP concentrations in antenatal hydronephrosisPubMed: 26096437
  • Intrarenal Renin-Angiotensin System Mediates Fatty Acid-Induced ER Stress in the KidneyPubMed: 26672616
  • Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via its antioxidant, anti‑inflammatory, and anti‑apoptotic activities.Pubmed:27082705
  • Preconditioned suppression of prolyl-hydroxylases attenuates renal injury but increases mortality in septic murine modelsPubmed:26908768
  • Kidney Injury Molecule Levels in Type 2 Diabetes MellitusPubmed:27132791
  • Protective Effect of Dihydromyricetin Against Lipopolysaccharide-Induced Acute Kidney Injury in a Rat Modelpmc:PMC4754091
  • Deficiency in Apoptosis-Inducing Factor Recapitulates Chronic Kidney Disease via Aberrant Mitochondrial HomeostasisPubmed:26822084
  • Once daily administration of the SGLT2 inhibitor, empagliflozin, attenuates markers of renal fibrosis without improving albuminuria in diabetic db/db micePubmed:27226136
  • Vitamins C and E Alleviate Nephrotoxicity-induced by Potassium Bromate in RatsBarakat812016JALSI28698.pdf
  • Synergic Effect of α-Mangostin on the Cytotoxicity of Cisplatin in a Cervical Cancer Model.pubmed:28053694
  • Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via itsantioxidant, anti‑inflammatory, and anti‑apoptotic activities.pubmed:27082705
  • Protective Effects of DHA-PC against Vancomycin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis in BALB/c Micepubmed:29254330
  • Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.pubmed:29027259
  • Urinary Kidney Injury Molecule-1 but Not Urinary Neutrophil Gelatinase Associated Lipocalin Is Increased after Short Maximal Exercise10.1159/000481179
  • Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats …Pubmed:29550811
  • Exploring the therapeutic mechanism of Desmodium styracifolium on oxalate crystal-induced kidney injuries using comprehensive approaches based on …Pubmed:29950996
  • Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic NephropathyPubmed:29642511
  • Modeling heart failure risk in diabetes and kidney disease: limitations and potential applications of transverse aortic constriction in high-fat-fed micePubmed:29443547
  • Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in RatsPubmed: 30251228
  • A Phage Display-Identified Peptide Selectively Binds to Kidney Injury Molecule-1(KIM-1) and Detects KIM-1-Overexpressing Tumors in vivo
  • Urinary neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, N-acetyl-β-D-glucosaminidase levels and mortality risk in septic patients with …Pubmed: 30393493
  • UPLC/MS-Based Metabolomics Investigation of the Protective Effect of Hydrogen Gas Inhalation on Mice with Calcium Oxalate-Induced Renal InjuryPubmed: 30381664
  • The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic NephropathyPubmed: 30504713
  • Urine markers of renal tubular injury in idiopathic membranous nephropathy: A cross sectional studyPubmed: 30684459
  • Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicinePubmed: 30871515
  • Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase InhibitorsDoi: BioMed Research International
  • Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascadePubmed: 31197431
  • Effect of Cucumis melo var. flexuosus leaves extract on renal oxidative injury and inflammation in diabetic male albino rats
  • Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixturesPubmed: 31639605
  • page Metabolomics study of fasudil on cisplatin-induced kidney injuryPubmed: 31670380
  • MiR-214-3p exacerbates kidney damages and inflammation induced by hyperlipidemic pancreatitis complicated with acute renal injuryPubmed: 31790686
  • Protective effect of saxagliptin against renal ischaemia reperfusion injury in ratsPubmed: 31979992
  • Delineating a role for the Mitochondrial Permeability Transition Pore in Diabetic Kidney Disease by targeting Cyclophilin DPubmed: 31943002
  • Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathwayPubmed: 32798557
  • Serum NGAL, KIM-1, IL-18, L-FABP: new biomarkers in the diagnostics of acute kidney injury (AKI) following invasive cardiology proceduresPubmed: 32557377
  • Can Serum Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Help in Decision Making for Surgery in Antenatally Dedected …Pubmed: 33139211
  • Ratlarda sodyum florid ile oluşturulan toksikasyonda Karvakol'ün böbrekler üzerine etkisi
  • Rationale and design of the intervention in PHOspholamban RElated CArdiomyopathy STudy (i-PHORECAST)
  • Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers33423326
  • Value of urinary kidney injury molecule-1 levels in predicting acute kidney injury in very low birth weight preterm infants33372811
  • Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury33604384
  • Targeting Methylglyoxal in Diabetic Kidney Disease Using the Mitochondria-Targeted Compound MitoGamide33922959
  • Kim-1 Targeted Extracellular Vesicles: A New Therapeutic Platform for RNAi to Treat AKI34127536
  • Anti-oxidant impact of Lisinopril and Enalapril against acute kidney injury induced by doxorubicin in male Wistar rats: involvement of kidney injury molecule-133506137
  • Pentoxifylline treatment alleviates kidney ischemia/reperfusion injury: Novel involvement of galectin-3 and ASK-1/JNK & ERK1/2/NF-¦ÊB/HMGB-1 trajectories34030796
  • Could urinary kidney injury molecule-1 be a good marker in subclinical acute kidney injury in mild to moderate COVID-19 infection?34213713
  • Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental …34169407
  • Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structure34820603

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