Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay) 
KLa; Klotho Alpha
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 449.00 US$ 467.00 US$ 492.00 US$ 527.00 US$ 562.00 US$ 613.00 US$ 691.00 US$ 864.00
- Quantity
Overview
Properties
- Product No.LMH757Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathway
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-102 | 93 |
| EDTA plasma(n=5) | 79-90 | 83 |
| heparin plasma(n=5) | 87-94 | 90 |
| sodium citrate plasma(n=5) | 97-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-104% | 85-103% | 98-105% | 98-105% |
| EDTA plasma(n=5) | 94-105% | 92-99% | 84-98% | 81-88% |
| heparin plasma(n=5) | 98-105% | 81-88% | 95-103% | 86-101% |
| sodium citrate plasma(n=5) | 97-105% | 78-92% | 86-104% | 97-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:KL) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculaturePubmed: 24012609
- Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in MiceCom:Source
- α-Klotho is an acute phase protein and altered by restraint stress in micePubmed:Pmc4203207
- Les biomarqueurs des calcifications vasculairesPubMed: 26069069
- Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with KlothoPubMed: 25873020
- Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3PubMed: 25899975
- 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制Cn: Article
- The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanismPubMed: 26419931
- Soluble Klotho protein as a novel serum biomarker in patients with acromegalypmc:PMC4754356
- Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O PathwayPubmed:27174564
- Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice.pubmed:28387374
- Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management?pubmed:28429560
- Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patientsissn:2394-2916
- Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in miceISSN:1936-2625
- Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat modelpubmed:28943597
- Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion InjuryPubmed:29550818
- Advanced maternal age impairs spatial learning capacity in young adult mouse offspringPubmed:29636887
- Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complicationsPubmed:30042059
- Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal …Pubmed:30004089
- High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney diseasePubmed: 30189026
- The role of klotho in chronic kidney diseasePubmed: 30348110
- Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regenerationPubmed: 30451844
- Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndromePubmed: 30770217
- The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic ratsPubmed: 30801205
- CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protectionPubmed: 33223223
- Effects of salt intervention on serum levels of Klotho influenced by salt sensitivityPubmed: 33164306
- Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic ratsPubmed: 32744464
- Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic ratsPubmed: 32438076
- P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy.Pubmed: 32377919
- Ameliorating Effect of Klotho Protein on Rat Heart during I/R InjuryPubmed: 33123314
- Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With SchizophreniaPubmed: 32612508
- ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy33675964
- Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma CellsPubmed:35112226
- Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance
- Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome