Multiplex Assay Kit for Macrophage Migration Inhibitory Factor (MIF) ,etc. by FLIA (Flow Luminescence Immunoassay) 
GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 472.00 US$ 490.00 US$ 517.00 US$ 553.00 US$ 590.00 US$ 644.00 US$ 726.00 US$ 907.00
- Quantity
Overview
Properties
- Product No.LMA698Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokineInfection immunityRheumatologyAutoimmunity
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Macrophage Migration Inhibitory Factor (MIF) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Macrophage Migration Inhibitory Factor (MIF) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-99 | 91 |
| EDTA plasma(n=5) | 90-99 | 94 |
| heparin plasma(n=5) | 78-93 | 82 |
| sodium citrate plasma(n=5) | 89-103 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Macrophage Migration Inhibitory Factor (MIF) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 92-99% | 80-99% | 80-94% |
| EDTA plasma(n=5) | 79-96% | 86-99% | 96-105% | 82-105% |
| heparin plasma(n=5) | 86-98% | 83-101% | 79-104% | 95-103% |
| sodium citrate plasma(n=5) | 92-105% | 95-103% | 78-102% | 78-93% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:MIF) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Targeted reduction of advanced glycation improves renal function in obesityPubMed: 21412218
- Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the GutJimmunol: 5673
- Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver diseasePubMed: PMC3757527
- Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing micePubMed: PMC3466372
- Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetesPubmed:24957662
- The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the BladderPubMed: 25705692
- Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease.pubmed:28160092
- Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis pubmed:28919396
- Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic StrokePubmed:29335619
- Immune Response and Mechanisms of IFN-γ in Administration for KeratomycosisPubmed: 30307777
- Effect of voluntary running on expression of myokines in brains of rats with depressionPubmed: 30834799
- Biomarkers in Heart Failure and Associated Diseases
- Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvationPubmed: 31209799
- Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expressionPubmed: 31802473
- Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitisPubmed: 32062385
- Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population33850223
- Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytesPubmed:35115654