Multiplex Assay Kit for Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay) Rattus norvegicus (Rat) Multiplex ELISA

MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA705Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:MUC2) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.PubMed: 20717734
  • Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats.Pubmed:24627600
  • Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosisPubMed: 26217323
  • Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathwayPubMed: 26244071
  • Secreted mucins and airway bacterial colonization in non-CF bronchiectasisPubMed: 26172851
  • Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonizationdoi:10.1513
  • An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
  • Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA10.21769:BioProtoc.2879
  • The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung diseasePubmed: 30573494
  • Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast CancerPubmed: 30682816
  • PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific MetabolismPubmed: 31564549
  • Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic RatsPubmed: 32144600
  • Mucus layer modeling of human colonoids during infection with enteroaggragative E. coliPubmed: 32601325
  • Defective intestinal repair after short-term high fat diet due to loss of efferocytosis
  • Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis34145945
  • Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced …34370080

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