Multiplex Assay Kit for Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) 
Myelin A1 protein; Myelin membrane encephalitogenic protein
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 427.00 US$ 443.00 US$ 468.00 US$ 501.00 US$ 534.00 US$ 583.00 US$ 657.00 US$ 821.00
- Quantity
Overview
Properties
- Product No.LMA539Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityNeuro scienceAutoimmunity
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-93 | 83 |
| EDTA plasma(n=5) | 93-102 | 99 |
| heparin plasma(n=5) | 80-96 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-95% | 85-93% | 92-101% | 99-105% |
| EDTA plasma(n=5) | 81-93% | 97-105% | 94-105% | 92-101% |
| heparin plasma(n=5) | 80-92% | 96-103% | 92-104% | 85-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:MBP) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
- Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat BrainPubmed: 23183024
- Myelin basic protein and ischemia modified albumin levels in acute ischemic stroke casesPubMed: 26648996
- Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus)Pubmed:27165445
- Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
- Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord.pubmed:27573615
- Glial markers and emotional memory in rats following acute cerebral radiofrequency exposures.pubmed:27696165
- Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
- Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosispubmed:28489854
- Acrolein-mediated neurotoxicity in growing Wistar male ratsPubmed:30033014
- Association and Risk of Canine Distemper with Respect to Age, Sex and Breed of Dogs Suffering from Demyelinating Neuropathies10.5455:ijlr.20170811080455
- Biosensors and nanobiosensors for rapid detection of autoimmune diseases: a reviewPubmed: 31760523
- The role of light desynchronosis in the development of stress-induced aging
- Association of eosinophil-mediated inflammatory biomarkers with the presence of the Schatzki ring34091432
- Searching for Noninvasive Predictors of the Diagnosis and Monitoring of Eosinophilic Esophagitis¡ªThe Importance of Biomarkers of the Inflammatory Reaction?¡
- Monitoring autoimmune diseases by bioelectrochemical detection of autoantibodies. Application to the determination of anti-myelin basic protein autoantibodies in …Pubmed:35202838