Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) Cavia (Guinea pig ) Multiplex ELISA

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA879Gu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-103 84
EDTA plasma(n=5) 79-101 84
heparin plasma(n=5) 82-93 86
sodium citrate plasma(n=5) 86-103 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 94-101% 99-105% 78-102%
EDTA plasma(n=5) 83-97% 94-104% 79-90% 91-101%
heparin plasma(n=5) 79-101% 90-97% 88-99% 79-104%
sodium citrate plasma(n=5) 85-102% 99-105% 79-94% 92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:NPY) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney diseaseOnlinelibrary: hdi.12072
  • Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal childrenDegruyter: Source
  • The role of adipocytokines on depressive symptoms of patients with chronic kidney diseasePubmed: 23879728
  • The relationship of plasma neuropeptide Y levels with coronary collateral development.Pubmed: 24077326
  • The relationship of plasma neuropeptide Y levels with coronary collateral developmentLww:Source
  • Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonatePubmed:25623392
  • High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid HemorrhagePubMed: 25955865
  • High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate …Pubmed:26885531
  • Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetatepubmed:27604241
  • Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells.pubmed:27625336
  • Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor ratpubmed:27685769
  • Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axisPubmed:29850786
  • Salivary neuropeptides, stress, and periodontitisPubmed:28914594
  • Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factorPubmed:29685637
  • Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y …Pubmed: 30227410
  • Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome
  • Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome.
  • Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats.Pubmed: 32378074
  • Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double©\blind randomized?¡­33245168
  • Serotonin involvement in okadaic acid-induced diarrhoea in vivo34148100

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