Multiplex Assay Kit for Neurotrophin 3 (NT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

Matrices listed below were spiked with certain level of recombinant Neurotrophin 3 (NT3) and the recovery rates were calculated by comparing the measured value to the expected amount of Neurotrophin 3 (NT3) in samples.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotrophin 3 (NT3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotrophin 3 (NT3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurotrophin 3 (NT3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Anti-Neurotrophin 3 (NT3) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Neurotrophin 3 (NT3) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Neurotrophin 3 (NT3) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Neurotrophin 3 (NT3) in the sample and corresponding value of MFI (Median Fluorescence Intensity).