Multiplex Assay Kit for Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by FLIA (Flow Luminescence Immunoassay) 
Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 474.00 US$ 492.00 US$ 520.00 US$ 556.00 US$ 593.00 US$ 648.00 US$ 730.00 US$ 912.00
- Quantity
Overview
Properties
- Product No.LMB388Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunity
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-96 | 87 |
| EDTA plasma(n=5) | 80-95 | 80 |
| heparin plasma(n=5) | 98-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-99% | 79-93% | 91-103% | 89-99% |
| EDTA plasma(n=5) | 78-95% | 80-95% | 80-96% | 82-103% |
| heparin plasma(n=5) | 87-104% | 78-103% | 91-101% | 99-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:NGAL) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancyPubMed: PMC3794962
- Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 PathwayPubmed: 24369464
- Lipocalin-2: Response To Resistance Training in Obese Young MenIjssjournal: Source
- Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young MenTums: Source
- Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlapPubmed: 23794464
- Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathyPubmed:24802166
- Nephrotoxic effects of varenicline as the most effective drug used for smoking cessation: a preliminary experimental studyPubmed:25812822
- 血清及胆汁中NGAL 对于区分良恶性胆道狭窄的临床意义Wjgnet:Source
- Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty ratsSagepub:Source
- Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese MenBiomedpharmajournal:Source
- A Study On Thyroid Hormone And Its Effects On Exercise Ifcss:Source
- 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义Jcp-Sh:Source
- Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancyPubmed:Pmc4260273
- Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary diseasePubmed:Pmc4043430
- Impact of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal modelPubmed:24916275
- Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous CrisisPubmed:25448261
- Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitisPubMed: 26191125
- Increased cardiac index attenuates septic acute kidney injury: a prospective observational studyPubMed: 25745357
- 中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义Journal: Fulltext
- Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in RatsPubMed: 25909514
- Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal modelPubmed:26714833
- From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.Pubmed:26991748
- Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu
- The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in ratspubmed:27430552
- Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis.pubmed:27775820
- Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN)pubmed:28659153
- From varices to venous ulceration: the story of chronic venous disease described by metalloproteinasespubmed:26991748
- Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.pubmed:29027259
- Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac SurgeryPubmed:29386545
- Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activityPubmed:30036488
- Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic NephropathyPubmed:29642511
- Improved approach for normothermic machine perfusion of cold stored kidney graftsPubmed:30018731
- Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypesISBN:978-951-51-4039-5
- Potential Novel Biomarkers of Obstructive Nephropathy in Children with HydronephrosisDoi: 10.1155/2018/1015726
- The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic NephropathyPubmed: 30504713
- Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicinePubmed: 30871515
- Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascadePubmed: 31197431
- A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activationPubmed: 31447572
- Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixturesPubmed: 31639605
- Alkaline phosphatase treatment of Acute Kidney injury in an infant piglet Model of cardiopulmonary Bypass with Deep Hypothermic circulatory ArrestPubmed: 31578351
- The evaluation of acute kidney injury due to ischemia by urinary neutrophil gelatinase-induced lipocalin (uNGAL) measurement in patients who underwent partial …Pubmed: 32980930
- Upregulated miR-101 inhibits acute kidney injury–chronic kidney disease transition by regulating epithelial–mesenchymal transitionPubmed: 32633566
- CОСТОЯНИЕ ТИРЕОИДНОЙ ФУНКЦИИ У ПАЦИЕНТОВ С САХАРНЫМ ДИАБЕТОМ 1-ГО ТИПА И ХРОНИЧЕСКОЙ БОЛЕЗНЬЮ ПОЧЕК …
- Thyroid function in patients with type 1 diabetes mellitus and chronic kidney disease receiving renal replacement therapy
- Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers33423326
- Nrf2 deficiency aggravates the kidney injury induced by subacute cadmium exposure in mice33398418
- Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury33604384
- Elevated plasma level of neutrophil gelatinase-associated lipocalin (NGAL) in patients with breast cancer34104101
- Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental …34169407
- Preservation of Mitochondrial Coupling and Renal Function by Controlled Oxygenated Rewarming of Porcine Kidney Grafts34944524
- SERS based Y-shaped aptasensor for early diagnosis of acute kidney injuryPubmed:35733690