Multiplex Assay Kit for Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) Pan-species (General) Multiplex ELISA

NA; NAd; Norepinephrine; Norepi

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA907Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 97
EDTA plasma(n=5) 79-101 89
heparin plasma(n=5) 91-98 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-97% 97-104% 80-99% 85-96%
EDTA plasma(n=5) 89-101% 82-101% 93-101% 89-96%
heparin plasma(n=5) 95-102% 82-92% 79-93% 87-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:NE) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.

Giveaways

Increment services

Citations

  • Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis PatientsPubmed: 23530612
  • Apelin-13 and APJ in paraventricular nucleus contribute to hypertension via sympathetic activation and vasopressin release in spontaneously hypertensive ratsPubmed:24995933
  • Renal Sympathetic Denervation Improves Cardiac DysfunctionPubMed: 25804096
  • Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of 伪1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta.PubMed: 26025460
  • Catha edulis chewing effects on treatment of paranoid schizophrenic patientsPubMed: 25926735
  • Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of α1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aortacontent:21
  • Relaxin in paraventricular nucleus contributes to sympathetic overdrive and hypertension via PI3K-Akt pathwayPubmed:26746861
  • Determination of 17β-estradiol and noradrenaline in dog serum using surface-enhanced Raman spectroscopy and random Forestscience:S0026265X16300388
  • Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesitypubmed:27694818
  • Artepillin C, a Typical Brazilian Propolis-Derived Component, Induces Brown-Like Adipocyte Formation in C3H10T1/2 Cells, Primary Inguinal White Adipose Tissue-Derived Adipocytes, and Mice.pubmed:27598888
  • Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathypubmed:27889753
  • Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model.pubmed:28070608
  • c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expressionpubmed:27439062
  • Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in ratsS0899900716302350
  • Stress hormonal changes in the brain and plasma after acute noise exposure in mice.pubmed:27496010
  • Effects of renal denervation on monocrotaline induced pulmonary remodelingpubmed:28187460
  • Renal sympathetic denervation attenuates hypertension and vascular remodeling in renovascular hypertensive ratspubmed:27742806
  • Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats pubmed:28241996
  • Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload pubmed:27575955
  • cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesisPubmed:29784793
  • Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor …Pubmed:29854627
  • Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trialPubmed:29512230
  • A novel sympathetic neuronal GABA ergic signaling system regulates NE release to prevent ventricular arrhythmias after acute myocardial infarction
  • Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles
  • Noninvasive Stereotactic Radiotherapy for Renal Denervation in a Swine ModelPubmed: 31558254
  • Exacerbated pressor and sympatho-excitatory effects of central Elabela in spontaneously hypertensive ratsPubmed: 31834836
  • Resiniferatoxin reduces ventricular arrhythmias in heart failure via selectively blunting cardiac sympathetic afferent projection into spinal cord in ratsPubmed: 31811858
  • A porcine model for studying the cardiovascular consequences of high‐thoracic spinal cord injuryPubmed: 31876952
  • Sympathetic Nerves Positively Regulate Eosinophil-Driven Allergic Conjunctivitis via α1-Adrenergic Receptor SignalingPubmed: 32194050
  • Role of melatonin in intestinal mucosal injury induced by restraint stress in micePubmed: 32298156
  • Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axisPubmed: 33177714
  • Laparoscopic-based perivascular renal sympathetic nerve denervation: a feasibility study in a porcine modelPubmed: 32552871
  • Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammationPubmed: 32332859
  • Baroreceptor denervation reduces inflammatory status and worsens cardiovascular collapse during systemic inflammation
  • Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair …
  • Fractionation and bioassay-guided isolation of antihypertensive components of senecio serratuloides
  • Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-233352230
  • The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice33303990
  • Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D33549845
  • Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­33762821
  • The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models33884205
  • Renal interstitial fibrosis is reduced in high-fat diet-induced obese pigs following renal denervation from the intima and adventitia of the renal artery34852339
  • Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy …Pubmed:35189708
  • Autonomic nervous system receptor-mediated regulation of mast cell degranulation modulates the inflammation after corneal epithelial abrasionPubmed:35421396

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