Multiplex Assay Kit for Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay) Rattus norvegicus (Rat) Multiplex ELISA

TNFAIP5; TSG-14; Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMK411Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 96
EDTA plasma(n=5) 95-102 99
heparin plasma(n=5) 92-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-95% 93-102% 97-105% 88-98%
EDTA plasma(n=5) 78-102% 94-101% 82-105% 95-102%
heparin plasma(n=5) 80-99% 79-105% 80-91% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PTX3) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Pentraxin 3, Long (PTX3) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis ModelPubMed: 22966213
  • Effect of Vagus Nerve Stimulation on Focal Transient Cerebral Ischemia and Reperfusion in Adult Male White New Zealand RabbitsSfu: Source
  • Hemodialysis-induced changes in the blood composition affect function of the endotheliumOnlinelibrary: hdi.12148
  • Hemodialysis‐induced changes in the blood composition affect function of the endotheliumPubmed:24612443
  • The effects of sunitinib on endometriosisPubmed:25093747
  • Exenatide improves ovarian and endometrial injury and preserves ovarian reserve in streptozocin induced diabetic ratsPubmed:25366063
  • Ovarian failure in diabetic rat model: Nuclear factor-kappaB, oxidative stress, and pentraxin-3Pubmed:25510691
  • Octreotide protects ovary against ischemia–reperfusion injury in rats: Evaluation of histological and biochemical parametersPubMed: 26223286
  • The long pentraxin-3 is a useful marker for diagnosis of ovarian torsion: An experimental rat modelPubMed: 26470829
  • Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers5952149
  • Study on Association of Pentraxin 3 and Diabetic Nephropathy in a Rat ModelPubmed:29725602
  • The Relationship between Serum Pentraxin-3 Levels and Severity of Coronary Heart DiseasepaperID:86206
  • Serum levels and gene expression of pentraxin 3 are elevated in COPDPubmed: 30572222
  • Pentraxin 3 Detects Clinically Significant Fibrosis in Patients with Chronic Viral Hepatitis C
  • Jie Du Tong Ye San Prevents N-Nitrosomethylbenzylamine-Induced Esophageal Carcinogenesis via Inhibition of Inflammation and Proliferation
  • Study on Serum Pentraxin-3 Levels in Vasculitis with HypertensionPubmed: 31264920
  • The role of CRP and Pentraxin 3 in the prediction of systemic inflammatory response syndrome and death in acute pancreatitisPubmed: 31798002
  • Losartan ameliorates ovarian ischaemia/reperfusion injury in rats: an experimental studyPubmed: 31955629
  • Effects of Methylene Blue on Ovarian TorsionLDetorsion Injury in a Rat ModelPubmed: 32593749
  • Therapeutic effects of vitamin D on acetic acid-induced colitis in ratsPubmed: 32555936
  • Pentraxin 3 as a marker for cardiovascular disease risk in overweight and obese children33716459
  • Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­34035808
  • High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese …34684637
  • Protective effects of dichloroacetic acid on endometrial injury and ovarian reserve in an experimental rat model of diabetes mellitus34595798

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