Multiplex Assay Kit for Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) Homo sapiens (Human) Multiplex ELISA

OSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMH339Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-102 92
EDTA plasma(n=5) 95-104 101
heparin plasma(n=5) 97-105 102
sodium citrate plasma(n=5) 80-96 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 81-96% 84-99% 86-95%
EDTA plasma(n=5) 89-102% 87-94% 89-97% 96-103%
heparin plasma(n=5) 84-102% 83-90% 79-103% 81-89%
sodium citrate plasma(n=5) 94-101% 94-101% 98-105% 84-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:POSTN) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Periostin (POSTN) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • Circulating Periostin Is Elevated In Patients With Hemoglobinopathies and Correlates With Bone Mineral Density In Double Heterozygous Sickle-Cell/Beta-Thalassemia Patients; A Novel Marker Of Bone Strength?Bloodjournal: Source
  • Serum periostin is associated with fracture risk in postmenopausal women: a 7 years prospective analysis of the OFELY studyPubmed: 24628551
  • Serum Periostin Is Associated With Fracture Risk in Postmenopausal Women: A 7-Year Prospective Analysis of the OFELY StudyPubmed:24628551
  • Circulating periostin levels in patients with AS: association with clinical and radiographic variables, inflammatory markers and molecules involved in bone formationPubmed:25349442
  • Assessment of periostin levels in serum and gingival crevicular fluid of patients with periodontal diseasePubmed:25529858
  • 血清内脂素水平与多囊卵巢综合征的相关性研究Xinyixue:Source
  • Serum periostin is associated with prevalent knee osteoarthritis and disease incidence/progression in women: the OFELY studyPubMed: 26072384
  • Serum periostin is a potential biomarker for non-alcoholic fatty liver disease: a case– control studyPubMed: 26362060
  • High levels of periostin correlate with increased fracture rate, diffuse MRI pattern, abnormal bone remodeling and advanced disease stage in patients with newly …pubmed:27716740
  • Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity.pubmed:28521873
  • Circulating periostin levels increase in association with bone density loss and healing progression during the early phase of hip fracture in Chinese older womenpubmed:28382553
  • The Utility of Biomarkers in Osteoporosis Managementpubmed:28271451
  • Cortical and trabecular bone microstructure did not recover at weight-bearing skeletal sites and progressively deteriorated at non-weight-bearing sites during the year following International Space Station missionsdoi:10.1002
  • Periostin and sclerostin levels in juvenile Paget's diseasepubmed:29263750
  • Development of a New Immunoassay for Human Cathepsin K-Generated Periostin Fragments as a Serum Biomarker for Cortical Bonepubmed:28725907
  • Characterization of a sandwich ELISA for the quantification of all human periostin isoformspubmed:28493527
  • Interaction between LRP5 and periostin gene polymorphisms on serum periostin levels and cortical bone microstructurepubmed:29038835
  • Effect of age and gender on serum periostin: Relationship to cortical measures, bone turnover and hormonespubmed:28323143
  • Association between serum periostin concentrations and outcome after acute spontaneous intracerebral hemorrhagepubmed:28882488
  • Serum periostin concentrations and outcomes after severe traumatic brain injurypubmed:28668564
  • Predictive and prognostic value of serum periostin in advanced non–small cell lung cancer patients receiving chemotherapypubmed:28459197
  • Serum Levels of a Cathepsin-K Generated Periostin Fragment Predict Incident Low-Trauma Fractures in Postmenopausal Women Independently of BMD and FRAXpubmed:28766739
  • ОСОБЕННОСТИ СОСТОЯНИЯ ЗДОРОВЬЯ НОВОРОЖДЕННЫХ, РОЖДЕННЫХ ОТ МАТЕРЕЙ С БРОНХИАЛЬНОЙ АСТМОЙ2017; 62:(4)
  • Circulating periostin in patients with nonalcoholic fatty liver disease.pubmed:27738886
  • Periostin cross‑reacts with the renin‑angiotensin system during liver fibrosis developmentpubmed:28849131
  • Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in micePubmed:29929978
  • The C-Terminal Intact Forms of Periostin (iPTN) Are Surrogate Markers for Osteolytic Lesions in Experimental Breast Cancer Bone MetastasisPubmed:29916127
  • Increased serum periostin concentrations are associated with the presence of diabetic retinopathy in patients with type 2 diabetes mellitusPubmed:29349642
  • Rheumatoid arthritis in remission
  • Myostatin and other musculoskeletal markers in lung transplant recipientsPubmed: 30317402
  • Polycystic Ovary Syndrome is Associated with Elevated Periostin LevelsPubmed: 30372763
  • ИСПОЛЬЗОВАНИЕ СЫВОРОТОЧНОГО ПЕРИОСТИНА В КАЧЕСТВЕ МАРКЕРА ОБОСТРЕНИЙ АСТМЫ У ДЕТЕЙ
  • Tartrate-resistant acid phosphatase 5b, but not periostin, is useful for assessing Paget's disease of bonePubmed: 31051316
  • Identification and diagnostic value of pleural fluid periostin and serum periostin of malignant pleural effusions in patients with non–small‐cell lung cancerPubmed: 31268191
  • Serum periostin is associated with cancer mortality but not cancer risk in older home-dwelling men: A 8-year prospective analysis of the STRAMBO studyPubmed: 31812700
  • Fibronectin rescues aberrant phenotype of endothelial cells lacking either CCM1, CCM2 or CCM3Pubmed: 32515053
  • Serum and placental periostin levels in women with early pregnancy lossPubmed: 32460058
  • Evaluation of periostin levels in gingival crevicular fluid and peri-implant sulcus fluid in patients with periodontal and peri-implanter disease: A cross-sectional …
  • Myostatin and Markers of Bone Metabolism in Dermatomyositis
  • Periostin promotes arterial calcification through PPAR¦Ã-related glucose metabolism reprogramming33834866
  • High Blood Eosinophil and YKL-40 Levels, as Well as Low CXCL9 Levels, are Associated with Increased Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease33814903
  • Serum total periostin is an independent marker of overall survival in bone metastases of lung adenocarcinoma34150488
  • The Relationship between Serum Periostin Levels and Neutrophil¨CLymphocyte Ratio with Lesion Localization and Acute Prognosis in Acute Ischemic Stroke34137680
  • First Admission Neutrophil–Lymphocyte Ratio May Indicate Acute Prognosis of Ischemic Stroke34137680

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