Multiplex Assay Kit for Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay) Homo sapiens (Human) Multiplex ELISA

P1CP; C-Propeptide Of Type I Procollagen; Procollagen I Carboxy Terminal Propeptide

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA570Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 86-101 92
heparin plasma(n=5) 94-105 101
sodium citrate plasma(n=5) 87-101 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-89% 92-101% 96-104% 88-96%
EDTA plasma(n=5) 78-103% 89-96% 80-90% 93-102%
heparin plasma(n=5) 79-89% 90-103% 85-92% 86-102%
sodium citrate plasma(n=5) 84-93% 96-105% 94-101% 83-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PICP) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Procollagen I C-Terminal Propeptide (PICP) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Increment services

Citations

  • Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacityScienceDirect: S0899900709004717
  • Serum markers of deranged myocardial collagen turnover: their relation to malignant ventricular arrhythmias in cardioverter-defibrillator recipients with heart failure.PubMed: 23067911
  • Circulating biomarkers of collagen metabolism in arterial hypertension: relevance of target organ damagePubmed: 23615327
  • Cdkn1a is a key mediator of rat pancreatic stellate cell senescencePubmed: 23719597
  • Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry diseasePubmed:25073565
  • An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized MicePubmed:25184758
  • The Anti-Osteoanabolic Function of Sclerostin is Blunted in Mice Carrying a High Bone Mass Mutation of Lrp5Pubmed:25640331
  • The active role of osteoporosis in the interaction between osteoblasts and bone metastasesPubMed: 26057367
  • Consumption of vitamin D2PubMed: 25792284
  • Bilaterale Kommunikation zwischen Osteoblasten und Osteoklastenvolltexte:2016
  • Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathypubmed:27516211
  • Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRRpubmed:28004175
  • The association between insulin-like growth factor 1 (IGF-1), IGF-binding proteins (IGFBPs), and the carboxyterminal propeptide of type I procollagen (PICP) in pre- and postmenopausal women with rheumatoid arthritis.pubmed:27775453
  • Effect of montelukast on markers of airway remodeling in children withasthma.pubmed:27657514
  • The association between insulin-like growth factor 1 (IGF-1), IGF-binding proteins (IGFBPs), and the carboxyterminal propeptide of type I procollagen (PICP) in pre- and postmenopausal women with rheumatoid arthritisloi:irhe20
  • Conditional Mouse Models Support the Role of SLC39A14 (ZIP14) in Hyperostosis Cranialis Interna and in Bone HomeostasisISBN: 978-94-6299-582-6
  • Endothelial‑to‑mesenchymal transition in human idiopathic dilated cardiomyopathypubmed:29115553
  • Age-related changes in biochemical bone profile in thalassemic childrenpubmed:28967496
  • Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR10.1007/s00380-016-0930-y
  • The Lrp4 R1170Q homozygous knock‐in mouse recapitulates the bone phenotype of sclerosteosis in humanspubmed:28477420
  • 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis.pubmed:28460256
  • Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events.pubmed:29120858
  • Mutations That Alter the Carboxy‐Terminal‐Propeptide Cleavage Site of the Chains of Type I Procollagen Are Associated With a Unique Osteogenesis Imperfecta …Pubmed:29669177
  • Assessment of myocardial fibrosis by late gadolinium enhancement imaging and biomarkers of collagen metabolism in chronic rheumatic mitral regurgitation.Pubmed:29443354
  • DNA methylation regulates α‐smooth muscle actin expression during cardiac fibroblast differentiationPubmed: 30362530
  • Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPDPubmed: 30655663
  • Low fibrosis biomarker levels predict cardiac resynchronization therapy responsePubmed: 30988339
  • Agonist-induced activation of the S1P receptor 2 constitutes a novel osteoanabolic therapy for the treatment of osteoporosis in micePubmed: 31028959
  • Deficiency of sphingosine-1-phosphate receptor 3 does not affect the skeletal phenotype of mice lacking sphingosine-1-phosphate lyasePubmed: 31314788
  • Mice lacking plastin-3 display a specific defect of cortical bone acquisitionPubmed: 31678489
  • Type 2 Diabetes Mellitus and Chronic Heart Failure with Midrange and Preserved Ejection Fraction: A Focus on Serum Biomarkers of FibrosisPubmed: 33224989
  • Piezo1 Inactivation in Chondrocytes Impairs Trabecular Bone FormationPubmed: 33180356
  • Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort
  • Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда
  • Early diagnosis of myocardial fibrosis in patients with epicardial obesity
  • Association between Markers of Fibrosis and Heart Failure Incidence in Patients with Type 2 Diabetes Mellitus34778465
  • Extra Cellular Matrix Deposition and Assembly in Dermis Spheroids34321901
  • Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy34296634
  • Effects of 8-week increment aerobic exercise program on bone metabolism and body composition in young non-athletesPubmed:35141785
  • Simple Predictors for Cardiac Fibrosis in Patients with Type 2 Diabetes Mellitus: The Role of Circulating Biomarkers and Pulse Wave VelocityPubmed:35628969

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