Multiplex Assay Kit for Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) Chicken (Gallus) Multiplex ELISA

LTH; Luteotropic Hormone

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA846Ga.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-101 94
EDTA plasma(n=5) 84-94 89
heparin plasma(n=5) 78-101 92
sodium citrate plasma(n=5) 94-105 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-99% 98-105% 80-89% 82-89%
EDTA plasma(n=5) 86-94% 87-98% 78-99% 90-97%
heparin plasma(n=5) 78-98% 80-102% 96-105% 88-96%
sodium citrate plasma(n=5) 91-98% 82-96% 94-102% 90-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PRL) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary GlandJcb: 39297
  • TLR9 expression is associated with prognosis in patients with glioblastoma multiformeScienceDirect: S0967586811003572
  • Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodesSciencedirect:S0925400514000720
  • Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifersPubmed:27051212
  • Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calvespublication:306322503
  • Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cellspubmed:29096715
  • The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicityPubmed:30021657
  • Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
  • The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistancePubmed:29551354
  • The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathwayPubmed:29388711
  • Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenograftsPubmed: 30334324
  • Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndromePubmed: 30404636
  • Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemiaPubmed: 30143940
  • Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride TreatmentPubmed: 32152847
  • Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattlePubmed: 32684470
  • Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
  • Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection34391195
  • Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer34439574
  • Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome MicePubmed:35057459

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