Multiplex Assay Kit for Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) Bos taurus; Bovine (Cattle) Multiplex ELISA

PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA929Bo.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-103 99
EDTA plasma(n=5) 78-90 87
heparin plasma(n=5) 80-92 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 86-99% 84-96% 92-101%
EDTA plasma(n=5) 89-99% 95-102% 87-101% 86-97%
heparin plasma(n=5) 87-105% 96-104% 91-105% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:RBP4) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 Liebert: 20100078
  • Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2PubMed: 21130593
  • Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in RatsPubMed: PMC3368504
  • Combined galanin with insulin improves insulin sensitivity of diabetic rat musclesPubmed:24501381
  • Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic ratsPubmed:25240061
  • Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic ratsPubmed:25445608
  • Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty RatsPubmed:25697126
  • Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndromePubMed: 26351173
  • Expression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazonePubmed:27446311
  • Expression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone.pubmed:27446311
  • Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosisS0021967317311858
  • Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysisPubmed:29386009
  • Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humansPubmed:29335416
  • RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathwayPubmed:30015949
  • Relationship between retinol and risk of diabetic retinopathy: a case-control study
  • Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattlePubmed: 31147589
  • Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals
  • Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma
  • The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren33575861
  • Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren33834274
  • Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats33833309
  • Biomarkers of cadmium exposure and renal function in estuarine adult villagers34773507

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