Multiplex Assay Kit for Secreted Frizzled Related Protein 5 (SFRP5) ,etc. by FLIA (Flow Luminescence Immunoassay) 
SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 437.00 US$ 454.00 US$ 479.00 US$ 512.00 US$ 546.00 US$ 596.00 US$ 672.00 US$ 840.00
- Quantity
Overview
Properties
- Product No.LMC842Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transduction
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Secreted Frizzled Related Protein 5 (SFRP5) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Secreted Frizzled Related Protein 5 (SFRP5) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 87-104 | 94 |
| EDTA plasma(n=5) | 98-105 | 101 |
| heparin plasma(n=5) | 97-105 | 101 |
| sodium citrate plasma(n=5) | 79-101 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Secreted Frizzled Related Protein 5 (SFRP5) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-104% | 94-101% | 97-104% | 81-93% |
| EDTA plasma(n=5) | 84-96% | 87-104% | 93-101% | 93-101% |
| heparin plasma(n=5) | 93-103% | 82-90% | 98-105% | 84-102% |
| sodium citrate plasma(n=5) | 88-102% | 92-99% | 99-105% | 96-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:SFRP5) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistancePubMed: 23290274
- Sfrp5 correlates with insulin resistance and oxidative stressPubMed: 23398169
- Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in childrenPubmed: 24603290
- SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in childrenPubmed: 24330025
- Circulating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humansPubmed: 23185036
- Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery diseasePubmed: 24530778
- Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammationPubmed:Pmc4072672
- Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammationPubmed:24840810
- Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinomaPubmed:25120720
- Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controlsPubmed:25139699
- The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsisPubmed:25382802
- Circulating SFRP5 levels are elevated in drug-naPubMed: 25139699
- Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levelsPubMed: 26100762
- Sfrp5 associates with beta-cell function in humansPubmed:27019073
- Bone marrow sFRP5 level is negatively associated with bone formation markerspubmed:27986984
- Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adultspubmed:28303637
- Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 studypubmed:28851362
- Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With …Pubmed:29674475
- Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican AmericansPubmed:29427376
- Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic ratsarticle-1-352-en.pdf
- The effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndromePubmed: 30194556
- Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling
- A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profilePubmed: 30375290
- Role of wnt5a in Metabolic Inflammation in HumansPubmed: 30137542
- Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea PatientsPubmed: 32300333
- Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive diseasePubmed: 33188773
- Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and …Pubmed: 32491086
- Secreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway34469882
- Association of serum uromodulin with adipokines in dependence of type 2 diabetes34920231
- Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort studyPubmed:34990470
- Investigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition …Pubmed:35475790
- Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop …