Multiplex Assay Kit for Secretory Immunoglobulin A (sIgA) ,etc. by FLIA (Flow Luminescence Immunoassay)
S-IgA
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 427.00 US$ 443.00 US$ 468.00 US$ 501.00 US$ 534.00 US$ 583.00 US$ 657.00 US$ 821.00
- Quantity
Overview
Properties
- Product No.LMA641Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityImmune moleculeReproductive scienceGenetic scienceDermatologyImmunodeficiency
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Recovery
Matrices listed below were spiked with certain level of recombinant Secretory Immunoglobulin A (sIgA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Secretory Immunoglobulin A (sIgA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 80-101 | 93 |
EDTA plasma(n=5) | 97-104 | 101 |
heparin plasma(n=5) | 85-94 | 89 |
sodium citrate plasma(n=5) | 80-92 | 89 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretory Immunoglobulin A (sIgA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretory Immunoglobulin A (sIgA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Secretory Immunoglobulin A (sIgA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 88-95% | 80-90% | 79-96% | 84-99% |
EDTA plasma(n=5) | 80-102% | 78-97% | 83-101% | 92-99% |
heparin plasma(n=5) | 97-105% | 82-94% | 85-102% | 89-98% |
sodium citrate plasma(n=5) | 82-101% | 80-99% | 96-105% | 89-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:sIgA) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
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- RESPON IMUN MUKOSA DAN SELULER PADA TIKUS YANG DIBERI BUBUK SUSU KAMBING DENGAN INFEKSI Salmonella Typhimurium [Mucosal and Cellular Immune Response of Rat Given Goat Milk Powder and Infected with Salmonella Typhimurium]Ipb: Source
- Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88Pubmed: 24570422
- Potency of Lactobacillus plantarum Dad-13 and Sweet Potato (Ipomoea batatas) Fiber as Immunomodulator in Rats Infected With Salmonella TyphimuriumCcsenet:Source
- Repeated Oronasal Exposure to Lipopolysaccharide Induced Mucosal IgA Responses in Periparturient Dairy CowsPubmed:25061754
- Dietary Apostichopus japonicus enhances the respiratory and intestinal mucosal immunity in immunosuppressive micePubmed:25186733
- Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic micePubmed:25308407
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- Role of Maternal Allergy on Immune Markers in Colostrum and Secretory Immunoglobulin A in Stools of Breastfed InfantsPubMed: 26243755
- Oropharyngeal Colostrum Administration in Extremely Premature Infants: An RCTPubMed: 25624376
- Salivary Secretory Immunoglobulin (SIgA) and Lysozyme in Malignant Tumor Patientsjournals:8701423
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- Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period.pubmed:28244338
- Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunologypubmed:28266682
- Saccharomyces boulardiiadministration can inhibit the formation of gastric lymphoid follicles induced by Helicobacter suis infectionpubmed:28115360
- Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying henspubmed:28938780
- Novel dietary polysaccharide SIP promotes intestinal secretory immunoglobulin A secretion in mice under chemotherapy10.1016/j.jff.2017.08.012
- Evaluation of Susceptibility and Innate Immune Response to Candida albicans in Mice with Sub-healthijp.2018.689.697
- Correlation of s-IgA and IL-6 Salivary with Caries Disease and Oral Hygiene Parameters in ChildrenPubmed: 31892186
- Fluorescence polarization-based rapid detection system for salivary biomarkers using modified DNA aptamers containing base-appended basesPubmed: 31855403
- Artesunate protects immunosuppression mice induced by glucocorticoids via enhancing pro-inflammatory cytokines release and bacterial clearancePubmed: 33045197
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