Multiplex Assay Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ,etc. by FLIA (Flow Luminescence Immunoassay) 
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 437.00 US$ 454.00 US$ 479.00 US$ 512.00 US$ 546.00 US$ 596.00 US$ 672.00 US$ 840.00
- Quantity
Overview
Properties
- Product No.LMP155Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transduction
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and the recovery rates were calculated by comparing the measured value to the expected amount of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 85-93 | 89 |
| heparin plasma(n=5) | 97-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-90% | 99-105% | 98-105% | 96-103% |
| EDTA plasma(n=5) | 85-93% | 86-95% | 88-95% | 83-103% |
| heparin plasma(n=5) | 99-105% | 78-101% | 79-94% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:WNT3A) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) in the sample and corresponding value of MFI (Median Fluorescence Intensity).
Giveaways
Increment services
Citations
- Wls promotes the proliferation of breast cancer cells via Wnt signalingPubmed:25801232
- Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIsPubMed: 26209081
- Gelatin device for the delivery of growth factors involved in endochondral ossificationpubmed:28380024
- Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.pubmed:28754618
- Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus MusclePubmed:30151071
- Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast CancerDoi: 10.15369/sujms.30.359
- Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular CarcinomaPubmed: 31737122
- Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infectionPubmed: 32920199
- An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties34823885