Mini Samples ELISA Kit for Atrial Natriuretic Peptide (ANP) Mus musculus (Mouse) Mini ELISA

ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin

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  • Mini Samples ELISA Kit for Atrial Natriuretic Peptide (ANP) Packages (Simulation)
  • Mini Samples ELISA Kit for Atrial Natriuretic Peptide (ANP) Packages (Simulation)
  • Mini Samples ELISA Kit for Atrial Natriuretic Peptide (ANP) Results demonstration
  • MEA225Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Atrial Natriuretic Peptide (ANP) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Atrial Natriuretic Peptide (ANP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-92 85
EDTA plasma(n=5) 95-104 101
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Atrial Natriuretic Peptide (ANP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Atrial Natriuretic Peptide (ANP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Atrial Natriuretic Peptide (ANP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 89-98% 80-89% 96-105%
EDTA plasma(n=5) 83-91% 90-103% 98-105% 92-101%
heparin plasma(n=5) 84-93% 78-103% 86-101% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Mini Samples ELISA Kit for Atrial Natriuretic Peptide (ANP)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Atrial Natriuretic Peptide (ANP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Atrial Natriuretic Peptide (ANP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Atrial Natriuretic Peptide (ANP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Atrial Natriuretic Peptide (ANP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Volume Status and Diuretic Therapy in Systolic Heart Failure and the Detection of Early Abnormalities in Renal and Tubular FunctionPubMed: 21616283
  • Release of atrial natriuretic peptide precedes shedding of the endothelial glycocalyx equally in patients undergoing on- and off-pump coronary artery bypass surgeryPubMed: 21769675
  • Atrial natriuretic Peptide, arginine vasopressin Peptide and cortisol serum levels in opiate-dependent patientsPubMed: 23406607
  • Alcohol-induced changes in methylation status of individual CpG sites, and serum levels of vasopressin and atrial natriuretic peptide in alcohol-dependent patients during detoxification treatment.Pubmed: 24356727
  • Hypervolemia increases release of atrial natriuretic peptide and shedding of the endothelial glycocalyxPubmed:25497357
  • Sodium-glucose co-transporter-2 inhibitors suppress atrial natriuretic peptide secretion in patients with newly diagnosed Type 2 diabetes.Pubmed:26927445
  • Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathwayPubmed:26970569
  • Blood Pressure and Atrial Natriuretic Peptide (ANP) Levels in Patients with Autosomal Dominant Polycystic Kidney Disease.papers:uobj_paper_2016_62613672.pdf
  • Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathypubmed:29240788
  • Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy.pubmed:29063791
  • SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylationPubmed:29504933
  • Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity andPubmed:29727991
  • Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in micePubmed:29929978
  • Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart DiseasePubmed:29392348
  • Signature-oriented investigation of the efficacy of multicomponent drugs against heart failurePubmed: 30230922
  • Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension …Pubmed: 30359461
  • ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma
  • Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure.
  • The poly (ADP-ribosyl) ation of BRD4 mediated by PARP1 promoted pathological cardiac hypertrophy34094834
  • Alarin moderated myocardial hypertrophy via inhibiting cyclic adenosine monophosphate/protein kinase A signaling pathway to attenuate autophagy34624429
  • Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stressPubmed:35218795

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