Mini Samples ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB) Rattus norvegicus (Rat) Mini ELISA

CK2; CK-MB; Creatine Kinase,Muscle/Brain

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  • Mini Samples ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB) Packages (Simulation)
  • Mini Samples ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB) Packages (Simulation)
  • Mini Samples ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB) Results demonstration
  • MEA479Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Creatine Kinase MB Isoenzyme (CKMB) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Creatine Kinase MB Isoenzyme (CKMB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-102 90
EDTA plasma(n=5) 80-95 88
heparin plasma(n=5) 78-93 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Creatine Kinase MB Isoenzyme (CKMB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Creatine Kinase MB Isoenzyme (CKMB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Creatine Kinase MB Isoenzyme (CKMB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 89-101% 80-89% 80-92%
EDTA plasma(n=5) 94-102% 82-103% 86-104% 79-103%
heparin plasma(n=5) 81-95% 96-103% 78-96% 91-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Mini Samples ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Creatine Kinase MB Isoenzyme (CKMB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Creatine Kinase MB Isoenzyme (CKMB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Creatine Kinase MB Isoenzyme (CKMB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Creatine Kinase MB Isoenzyme (CKMB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia–Reperfusion InjuryPubMed: 22814936
  • N-Acetylcysteine and Allopurinol Confer Synergy in Attenuating Myocardial Ischemia Injury via Restoring HIF-1α/HO-1 Signaling in Diabetic RatsPubMed: PMC3715528
  • Effects of morphine and sufentanil preconditioning against myocardial ischemic-reperfusion injury in rabbitsPubMed: 26629064
  • Diabetes blocks the cardioprotective effects of sevoflurane postconditioning by impairing Nrf2/Brg1/HO-1 signalingPubmed:26973173
  • Effect of levocarnitine on TIMP-1, ICAM-1 expression of rats with coronary heart disease and its myocardial protection effectPubmed:26972400
  • Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fractionpubmed:27924211
  • Peroxisome proliferator-activated receptor γ (PPARγ) mediates the protective effect of quercetin against myocardial ischemia-reperfusion injury via …PMC5209473
  • In Vivo Cardioprotective Effects and Pharmacokinetic Profile of N-Propyl Caffeamide AgainstIschemia Reperfusion Injury.pubmed:27480649
  • Cardiac biomarkers in dogs with visceral leishmaniasisS0301-732X2016000300004
  • Remote Limb Ischaemic Postconditioning Protects Against Myocardial Ischaemia/ Reperfusion Injury in Mice: Activation of JAK/STAT3-Mediated Nrf2-Antioxidant Signalling10.1159:481755
  • Morinda Citrifolia (Noni) and Low Dose Aspirin Prevent Apoptotic Cell Death and Oxidative Stress on Isoproterenol Induced Myocardial Infarction in RatsDOI: 10.5152/etd.2017.1732
  • Metformin attenuated endotoxin-induced acute myocarditis via activating AMPKpubmed:28410530
  • Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity andPubmed:29727991
  • GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic micePubmed:29605032
  • Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in ratsPubmed:29956781
  • Cyclic helix B peptide ameliorates acute myocardial infarction in mice by inhibiting apoptosis and inflammatory responses
  • Adiponectin Facilitates Postconditioning Cardioprotection through Both AMPK-Dependent Nuclear and AMPK-Independent Mitochondrial STAT3 ActivationPubmed: 32190173
  • Tissue suction-mediated gene transfer to the beating heart in micePubmed: 32027678
  • Deletion of Rap1 protects against myocardial ischemia/reperfusion injury through suppressing cell apoptosis via activation of STAT3 signalingPubmed: 32020680
  • Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injuryPubmed: 32705277
  • Hydrogen sulphide and nitric oxide cooperate in cardioprotection against ischemia/reperfusion injury in isolated rat heartPubmed: 32871779
  • Melatonin Attenuates Anoxia/Reoxygenation Injury by Inhibiting Excessive Mitophagy Through the MT2/SIRT3/FoxO3a Signaling Pathway in H9c2 CellsPubmed: 32546969
  • Biological Safety and Biodistribution of Chitosan NanoparticlesPubmed: 32340313
  • The mRNA expression of Il6 and Pdcd1 are predictive and protective factors for doxorubicin?induced cardiotoxicity33300057
  • Inactivation of TOPK Caused by Hyperglycemia Blocks Diabetic Heart Sensitivity to Sevoflurane Postconditioning by Impairing the PTEN/PI3K/Akt Signaling33986917
  • Long non-coding RNA MALAT1 modulates myocardial ischemia-reperfusion injury though the PI3K/Akt/eNOS pathway by sponging miRNA-133a-3p to target IGF1R …Pubmed:34968461
  • Hyperglycemia-Induced Overexpression of PH Domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) Compromises the Cardioprotective Effect of Ischemic …Pubmed:35715527
  • Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733Pubmed:35215383
  • Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrestPubmed:35495584
  • MicroRNA-503 Exacerbates Myocardial Ischemia/Reperfusion Injury via Inhibiting PI3K/Akt-and STAT3-Dependent Prosurvival Signaling PathwaysPubmed:35620576
  • Vitamin E Attenuates Cardiomyopathy Via Alleviation of Autophagic Stress

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