Mini Samples ELISA Kit for Cyclic Guanosine Monophosphate (cGMP) 
c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate
- UOM
- FOB US$ 669.00 US$ 955.00 US$ 4,298.00 US$ 8,118.00 US$ 66,850.00
- Quantity
Overview
Properties
- Product No.MEA577Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionMetabolic pathway
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Mini Samples Cyclic Guanosine Monophosphate (cGMP) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Cyclic Guanosine Monophosphate (cGMP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-99 | 91 |
| EDTA plasma(n=5) | 78-99 | 84 |
| heparin plasma(n=5) | 83-93 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Cyclic Guanosine Monophosphate (cGMP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cyclic Guanosine Monophosphate (cGMP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Cyclic Guanosine Monophosphate (cGMP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-103% | 96-104% | 82-104% | 82-91% |
| EDTA plasma(n=5) | 84-92% | 79-98% | 79-94% | 86-101% |
| heparin plasma(n=5) | 90-101% | 88-98% | 92-103% | 99-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×3mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×10mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Cyclic Guanosine Monophosphate (cGMP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Cyclic Guanosine Monophosphate (cGMP) and unlabeled Mini Samples Cyclic Guanosine Monophosphate (cGMP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Cyclic Guanosine Monophosphate (cGMP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Cyclic Guanosine Monophosphate (cGMP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Cyclic Guanosine Monophosphate (cGMP) in the sample.
Giveaways
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Citations
- Cardioprotective effects of baicalein on heart failure via modulation of Ca2PubMed: 26706290
- Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitroPubmed:26706290
- Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation09540105.2017.1306494
- Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic ratsPubmed: 30454888
- ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma
- iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine
- Atrial natriuretic peptide inhibits epithelial-mesenchymal transition (EMT) of bronchial epithelial cells through cGMP/PKG signaling by targeting Smad3 in a murine …Pubmed: 31496319
- Effect of cGMP-activated aquaporin 1 on TRPV4 in rats with allodynia induced by chronic compression of the dorsal root ganglionPubmed: 31790718
- Effect of tadalafil on penile nitric oxide synthase and corporal smooth muscle in rats under dutasteride treatmentPubmed: 32160825
- Epigallocatechin gallate mediated sandwich-like coating for mimicking endothelium with sustained therapeutic nitric oxide generation and heparin releasePubmed: 33143876
- Antihypertensive effects of allicin on spontaneously hypertensive rats via vasorelaxation and hydrogen sulfide mechanismsPubmed: 32480217
- The role of adrenergic and muscarinic receptors in stress-induced cardiac injury34245378
- A nitric oxide-eluting and REDV peptide-conjugated coating promotes vascular healingPubmed:35366606