- FOB US$ 605.00 US$ 864.00 US$ 3,888.00 US$ 7,344.00 US$ 60,480.00
- Product No.MEL154Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only
- DownloadInstruction Manual
- CategoryHuman gene deletion
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.6pg/mL.
This assay has high sensitivity and excellent specificity for detection of Mini Samples Fibroblast Growth Factor 15 (FGF15).
No significant cross-reactivity or interference between Mini Samples Fibroblast Growth Factor 15 (FGF15) and analogues was observed.
Sign into your account
Share a new citation as an author
Upload your experimental result
Please fill in the blank.
- Packages (Simulation)
- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Matrices listed below were spiked with certain level of recombinant Mini Samples Fibroblast Growth Factor 15 (FGF15) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Fibroblast Growth Factor 15 (FGF15) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Fibroblast Growth Factor 15 (FGF15) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Fibroblast Growth Factor 15 (FGF15) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Fibroblast Growth Factor 15 (FGF15) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×60µL||Assay Diluent A||1×6mL|
|Detection Reagent B||1×60µL||Assay Diluent B||1×6mL|
|TMB Substrate||1×4.5mL||Stop Solution||1×3mL|
|Wash Buffer (30 × concentrate)||1×10mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Fibroblast Growth Factor 15 (FGF15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Fibroblast Growth Factor 15 (FGF15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Fibroblast Growth Factor 15 (FGF15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Fibroblast Growth Factor 15 (FGF15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21PubMed: 25774553
- Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammationpubmed:27580383
- Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis.pubmed:28130274
- The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance10.1186/s12902-017-0211-5
- The ileal FGF15/19 to hepatic FGFR4 axis regulates liver regeneration after partial hepatectomy in micePubmed:29468415
- FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver TumorigenesisPubmed: 30745141
- Interactions between the gravitostat and the fibroblast growth factor system for the regulation of body weight
- Lactoferrin promotes bile acid metabolism and reduces hepatic cholesterol deposition by inhibiting the farnesoid X receptor (FXR)-mediated enterohepatic axisPubmed: 31626262
- Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activationPubmed: 33036625
- Si-Wu-Tang ameliorates fibrotic liver injury via modulating intestinal microbiota and bile acid homeostasis34736501
- Dynamics of the gut-liver axis in rats with varying fibrosis severityPubmed:35637968