Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP) Mus musculus (Mouse) Mini ELISA

Incretin hormone; Glucose Dependent Insulinotropic Peptide

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  • Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP) Packages (Simulation)
  • Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP) Packages (Simulation)
  • Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP) Results demonstration
  • MEA882Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Gastric Inhibitory Polypeptide (GIP) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Gastric Inhibitory Polypeptide (GIP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-96 88
EDTA plasma(n=5) 85-94 91
heparin plasma(n=5) 78-99 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Gastric Inhibitory Polypeptide (GIP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Gastric Inhibitory Polypeptide (GIP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Gastric Inhibitory Polypeptide (GIP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 81-101% 78-96% 81-97%
EDTA plasma(n=5) 96-104% 78-103% 81-98% 81-103%
heparin plasma(n=5) 91-98% 98-105% 88-102% 78-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×9mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
    And then add 25μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Gastric Inhibitory Polypeptide (GIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Gastric Inhibitory Polypeptide (GIP) and unlabeled Mini Samples Gastric Inhibitory Polypeptide (GIP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Gastric Inhibitory Polypeptide (GIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Gastric Inhibitory Polypeptide (GIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Gastric Inhibitory Polypeptide (GIP) in the sample.

Citations

  • The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlyingsignaling mechanisms in a rat model of diabetes mellitus.pubmed:27721485
  • The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats.pubmed:28840471
  • Preserving Duodenal-Jejunal (Foregut) Transit Does Not Impair Glucose Tolerance and Diabetes Remission Following Gastric Bypass in Type 2 Diabetes Sprague …Pubmed:29098544
  • Predictors of Effectiveness of Glucagon-Like Peptide-1 Receptor Agonist Therapy in Patients with Type 2 Diabetes and Obesity
  • Low AS160 and high SGK basal phosphorylation associates with impaired incretin profile and type 2 diabetes in adipose tissue of obese patientsPubmed: 31734225
  • The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in RatsPubmed: 31701411
  • Prediction scale of response to liraglutide therapy as the method for increase of treatment efficacy in type 2 diabetesPubmed:35251693

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