Mini Samples ELISA Kit for Homocysteine (HCy) 
- UOM
- FOB US$ 685.00 US$ 979.00 US$ 4,406.00 US$ 8,322.00 US$ 68,530.00
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Overview
Properties
- Product No.MED984Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Mini Samples Homocysteine (HCy) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Homocysteine (HCy) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-93 | 90 |
| EDTA plasma(n=5) | 86-102 | 95 |
| heparin plasma(n=5) | 83-94 | 89 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Homocysteine (HCy) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Homocysteine (HCy) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Homocysteine (HCy) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 78-89% | 78-94% | 85-92% |
| EDTA plasma(n=5) | 87-98% | 94-101% | 88-105% | 87-101% |
| heparin plasma(n=5) | 93-101% | 78-97% | 82-96% | 86-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Homocysteine (HCy) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Homocysteine (HCy) and unlabeled Mini Samples Homocysteine (HCy) (Standards or samples) with the pre-coated antibody specific to Mini Samples Homocysteine (HCy). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Homocysteine (HCy) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Homocysteine (HCy) in the sample.
Giveaways
Increment services
Citations
- Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspringPubmed:29582727
- Homocysteinemia in relation to anemia in hypothyroid patientsDoi: 10.4103/sjamf.sjamf_31_18
- Organochlorine pesticides exposure may disturb homocysteine metabolism in pregnant womenPubmed: 31787282
- Baicalin rescues hyperglycemia-induced neural tube defects via targeting on retinoic acid signalingPubmed: 32774702
- Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China
- Homocysteinaemia in Heart Failure Patients in North Eastern Nigeria
- KARD?YOVASK¨¹LER S?STEM HASTALIKLARINDA R?SK FAKT?R¨¹ OLAN H?PERHOMOS?STE?NEM?'N?N POL?K?ST?K OVER SENDROMUNDAK? YER?
- Folate, Vitamin B12, and Homocysteine Levels in Women With Neural Tube Defect-Affected Pregnancy in Addis Ababa, EthiopiaPubmed:35464038