Mini Samples ELISA Kit for Hydroxyproline (Hyp) Pan-species (General) Mini ELISA

(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)

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  • Mini Samples ELISA Kit for Hydroxyproline (Hyp) Packages (Simulation)
  • Mini Samples ELISA Kit for Hydroxyproline (Hyp) Packages (Simulation)
  • Mini Samples ELISA Kit for Hydroxyproline (Hyp) Results demonstration
  • MEA621Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Mini Samples Hydroxyproline (Hyp) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Hydroxyproline (Hyp) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-94 84
EDTA plasma(n=5) 88-96 92
heparin plasma(n=5) 88-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Hydroxyproline (Hyp) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Hydroxyproline (Hyp) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Hydroxyproline (Hyp) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-91% 78-90% 86-94% 78-103%
EDTA plasma(n=5) 78-99% 78-94% 87-101% 93-102%
heparin plasma(n=5) 81-103% 83-102% 84-92% 86-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×9mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
    And then add 25μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

Mini Samples ELISA Kit for Hydroxyproline (Hyp)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Hydroxyproline (Hyp) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Hydroxyproline (Hyp) and unlabeled Mini Samples Hydroxyproline (Hyp) (Standards or samples) with the pre-coated antibody specific to Mini Samples Hydroxyproline (Hyp). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Hydroxyproline (Hyp) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Hydroxyproline (Hyp) in the sample.

Citations

  • Spleen-Derived Interleukin-10 Downregulates the Severity of High-Fat Diet-Induced Non-Alcoholic Fatty Pancreas DiseasePubMed: PMC3532347
  • Prediction of fibrosis in hepatitis C patients: assessment using hydroxyproline and oxidative stress biomarkersSpringer: Source
  • Royal jelly affects collagen crosslinking in bone of ovariectomized ratsSciencedirect:S1756464614000346
  • Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transitionPubMed: 25911951
  • Theophylline, an old drug with multi-faceted effects: Its potential benefits in immunological liver injury in ratsPubMed: 26165752
  • Effects of 4% Icodextrin on Experimental Spinal Epidural Fibrosispdf:JTNEPUB_15079_online.pdf
  • Celecoxib attenuates depressive-like behavior associated with immunological liver injury inC57BL/6 mice through TNF-α and NF-κb dependent mechanisms.pubmed:27580523
  • Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with …doi.org:10.4174
  • Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbitsPubmed:29932250
  • Renin–angiotensin system inhibition ameliorates CCl-induced liver fibrosis in mice through the inactivation of nuclear transcription factor kappa B10.1139:cjpp-2017-0728
  • Pirfenidone suppresses bleomycin‑induced pulmonary fibrosis and periostin expression in rats10.3892:etm.2018.6378
  • Therapeutic effect of hepatocyte growth factor-overexpressing bone marrow-derived mesenchymal stem cells on CCl 4-induced hepatocirrhosis.
  • The effects of platelet‐rich‐plasma gel application to the colonic anastomosis in hyperthermic intraperitoneal chemotherapy: An experimental rat modelPubmed: 31448554
  • Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungsPubmed: 32146192
  • Is platelet-rich plasma improves the anastomotic healing in hyperthermic intraperitoneal chemotherapy with oxaliplatin: an experimental rat studyPubmed: 32051817
  • Protective Effects of Contractubex® on Stricture Formation after Experimental Corrosive Esophageal Burns in RatsPubmed: 32654881
  • Effect of Oral Channa striatus in Laparotomy Wound Healing of Malnourished Rats.
  • Handgrip Strength and Vitamin D as Predictors of Liver Fibrosis and Malnutrition in Chronic Hepatitis C Patients33884041
  • Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies33999928
  • Curdione and Schisandrin C Synergistically Reverse Hepatic Fibrosis via Modulating the TGF-β Pathway and Inhibiting Oxidative Stress34858986

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