Mini Samples ELISA Kit for Nuclear Factor Kappa B (NFkB) Mus musculus (Mouse) Mini ELISA

NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit

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  • Mini Samples ELISA Kit for Nuclear Factor Kappa B (NFkB) Packages (Simulation)
  • Mini Samples ELISA Kit for Nuclear Factor Kappa B (NFkB) Packages (Simulation)
  • Mini Samples ELISA Kit for Nuclear Factor Kappa B (NFkB) Results demonstration
  • MEB824Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Nuclear Factor Kappa B (NFkB) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Nuclear Factor Kappa B (NFkB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-90 81
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 84-105 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Nuclear Factor Kappa B (NFkB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Nuclear Factor Kappa B (NFkB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Nuclear Factor Kappa B (NFkB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-97% 82-96% 88-102% 95-104%
EDTA plasma(n=5) 85-103% 83-101% 88-101% 82-98%
heparin plasma(n=5) 92-103% 91-99% 80-91% 80-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Mini Samples ELISA Kit for Nuclear Factor Kappa B (NFkB)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Nuclear Factor Kappa B (NFkB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Nuclear Factor Kappa B (NFkB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Nuclear Factor Kappa B (NFkB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Nuclear Factor Kappa B (NFkB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Surgical Removal of Endometrioma Decreases the NF-kB1 (p50/105) and NF-kB p65 (Rel A) Expression in the Eutopic Endometrium During the Implantation WindowSagepub: Source
  • Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with TNBS-induced recurrent ulcerative colitisPubMed: 21642017
  • New Method to Isolate and Culture Rat Kupffer CellsPubMed: PMC3743898
  • Long-term aspirin pretreatment in the prevention of cerulein-induced acute pancreatitis in ratsPubMed: PMC3660814
  • Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κBPubMed: PMC3570159
  • Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-ReperfusionPubmed:24816434
  • Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitisPubmed:24992835
  • Long-term warfarin therapy and biomarkers for osteoporosis and atherosclerosisPubMed: 26674156
  • Serological Analysis of Alpha-synuclein and NF-κB in Parkinson’s Disease PatientsPubMed: 26155470
  • Hysteroscopic polypectomy decreases NF-κB1 expression in the mid-secretory endometrium of women with endometrial polypPubMed: 25898371
  • Plasma nuclear factor kappa B and serum peroxiredoxin 3 in early diagnosis of hepatocellular carcinoma.PubMed: 25743848
  • Preventive use of Lactobacillus plantarum LS/07 and inulin to relieve symptoms of acute colitis.PubMed: 26345092
  • Use Of The Prebiotic Inulin In The Prevention Of Adverse Signs Of Acute ColitisView: J
  • Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the RatPubMed: 26700436
  • L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation PatientsPubMed: 26518955
  • Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and CytokinePubmed:27061742
  • THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATSpublication:298838934
  • Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathwaydoi:10.1111
  • Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms.pubmed:27777064
  • Forskolin, a hedgehog signalling inhibitor, attenuates carbon tetrachloride-induced liver fibrosis in rats.pubmed:27590029
  • Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms.pubmed:27915041
  • S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase.pubmed:27887948
  • Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms.pubmed:28303372
  • Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat.pubmed:28199883
  • Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation.pubmed:28033582
  • Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1)pubmed:28974454
  • Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiotapubmed:28713364
  • The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialispubmed:29137729
  • In vivo cellular and molecular gastroprotective mechanisms of chrysin; Emphasis on oxidative stress, inflammation and angiogenesispubmed:29126792
  • Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus.pubmed:28553224
  • Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus.pubmed:29256845
  • Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanismspubmed:28061403
  • Scutellarin alleviates lipopolysaccharide-induced cognitive deficits in the rat: Insights into underlying mechanisms.pubmed:29190543
  • Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic ratsPubmed:30026088
  • Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y CellsPubmed:29869772
  • Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the ratPubmed:29518393
  • Shock waves increase pulmonary vascular leakage, inflammation, oxidative stress, and apoptosis in a mouse modelPubmed:29984607
  • Anti-inflammatory potential of Lactobacillus plantarum LS/07 in acute colitis in rats10.2478:acve-2018-0005
  • HMGB1-RAGE signaling pathway in pPROMPubmed:29673663
  • Improvement of colitis by LS/07 and inulin10.2754:avb201786040399
  • The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4Pubmed:29760339
  • Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic ratsPubmed: 30205096
  • Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent mannerPubmed: 30195035
  • Effect of Dachaihu Decoction on non-alcoholic fatty liver disease model rats induced by a high-fat high-sugar diet
  • Amitriptyline attenuates bleomycin-induced pulmonary fibrosis: modulation of the expression of NF-κβ, iNOS, and Nrf2Pubmed: 30474696
  • Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metforminPubmed: 30513216
  • Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments
  • Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting MetastasisPubmed: 30651572
  • Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brainPubmed: 30881100
  • Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian …Pubmed: 31600920
  • Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cellsPubmed: 31916655
  • New insights into the protection of growth hormone in cisplatin-induced nephrotoxicity: The impact of IGF-1 on the Keap1-Nrf2/HO-1 signalingPubmed: 32209424
  • Effects of lncRNA gm4419 on rats with hypertensive cerebral atherosclerosis through NF-κB pathwayPubmed: 31858567
  • Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrheaPubmed: 33217518
  • Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cellsPubmed: 33206337
  • Effects of combined treatment with Indomethacin and Juglone on AOM/DSS induced colon carcinogenesis in Balb/c mice: Roles of inflammation and …Pubmed: 33148421
  • Morroniside attenuates apoptosis and pyroptosis of chondrocytes and ameliorates osteoarthritic development by inhibiting NF-κB signalingPubmed: 33022338
  • Examination of androgenetic alopecia with serum biomarkersPubmed: 32969583
  • Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying MechanismsPubmed: 32526752
  • UPLC-Orbitrap HRMS metabolic profiling of Cymbopogon citratus cultivated in Egypt; neuroprotective effect against AlCl3-induced neurotoxicity in ratsPubmed: 32376365
  • Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male ratsPubmed: 33152939
  • Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes MellitusPubmed: 33123597
  • Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
  • Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells33675893
  • Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats33882726
  • Masseter muscle and gingival tissue inflammatory response following treatment with high©\fructose corn syrup in rats: Anti©\inflammatory and antioxidant effects of kefir33864286
  • The Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway Regulation
  • Vitamin D supplementation inhibits NF-kß signaling pathway in lean and obese women with PCOSPubmed:35731068
  • Effect of subacute poisoning with lambdacyhalothrin on vascular endothelial growth factor 2 receptor in mice kidneysPubmed:35643467
  • Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model …Pubmed:35204140
  • Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid …

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