Mini Samples ELISA Kit for Pentraxin 3, Long (PTX3) Homo sapiens (Human) Mini ELISA

TNFAIP5; TSG-14; Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein

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  • Mini Samples ELISA Kit for Pentraxin 3, Long (PTX3) Packages (Simulation)
  • Mini Samples ELISA Kit for Pentraxin 3, Long (PTX3) Packages (Simulation)
  • Mini Samples ELISA Kit for Pentraxin 3, Long (PTX3) Results demonstration
  • MEK411Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Pentraxin 3, Long (PTX3) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Pentraxin 3, Long (PTX3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-95 91
EDTA plasma(n=5) 80-92 88
heparin plasma(n=5) 88-101 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Pentraxin 3, Long (PTX3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Pentraxin 3, Long (PTX3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Pentraxin 3, Long (PTX3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 84-104% 94-103% 81-96%
EDTA plasma(n=5) 79-96% 98-105% 83-101% 94-103%
heparin plasma(n=5) 83-93% 95-105% 84-104% 94-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Mini Samples ELISA Kit for Pentraxin 3, Long (PTX3)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Pentraxin 3, Long (PTX3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Pentraxin 3, Long (PTX3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Pentraxin 3, Long (PTX3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Pentraxin 3, Long (PTX3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis ModelPubMed: 22966213
  • Effect of Vagus Nerve Stimulation on Focal Transient Cerebral Ischemia and Reperfusion in Adult Male White New Zealand RabbitsSfu: Source
  • Hemodialysis-induced changes in the blood composition affect function of the endotheliumOnlinelibrary: hdi.12148
  • Hemodialysis‐induced changes in the blood composition affect function of the endotheliumPubmed:24612443
  • The effects of sunitinib on endometriosisPubmed:25093747
  • Exenatide improves ovarian and endometrial injury and preserves ovarian reserve in streptozocin induced diabetic ratsPubmed:25366063
  • Ovarian failure in diabetic rat model: Nuclear factor-kappaB, oxidative stress, and pentraxin-3Pubmed:25510691
  • Octreotide protects ovary against ischemia–reperfusion injury in rats: Evaluation of histological and biochemical parametersPubMed: 26223286
  • The long pentraxin-3 is a useful marker for diagnosis of ovarian torsion: An experimental rat modelPubMed: 26470829
  • Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers5952149
  • Study on Association of Pentraxin 3 and Diabetic Nephropathy in a Rat ModelPubmed:29725602
  • The Relationship between Serum Pentraxin-3 Levels and Severity of Coronary Heart DiseasepaperID:86206
  • Serum levels and gene expression of pentraxin 3 are elevated in COPDPubmed: 30572222
  • Pentraxin 3 Detects Clinically Significant Fibrosis in Patients with Chronic Viral Hepatitis C
  • Jie Du Tong Ye San Prevents N-Nitrosomethylbenzylamine-Induced Esophageal Carcinogenesis via Inhibition of Inflammation and Proliferation
  • Study on Serum Pentraxin-3 Levels in Vasculitis with HypertensionPubmed: 31264920
  • The role of CRP and Pentraxin 3 in the prediction of systemic inflammatory response syndrome and death in acute pancreatitisPubmed: 31798002
  • Losartan ameliorates ovarian ischaemia/reperfusion injury in rats: an experimental studyPubmed: 31955629
  • Effects of Methylene Blue on Ovarian TorsionLDetorsion Injury in a Rat ModelPubmed: 32593749
  • Therapeutic effects of vitamin D on acetic acid-induced colitis in ratsPubmed: 32555936
  • Pentraxin 3 as a marker for cardiovascular disease risk in overweight and obese children33716459
  • Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­34035808
  • High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese …34684637
  • Protective effects of dichloroacetic acid on endometrial injury and ovarian reserve in an experimental rat model of diabetes mellitus34595798

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