Mini Samples ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)
ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3
- UOM
- FOB US$ 607.00 US$ 867.00 US$ 3,902.00 US$ 7,370.00 US$ 60,690.00
- Quantity
Overview
Properties
- Product No.MEB684Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionTumor immunityImmune moleculeHepatology
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Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 78-97 | 93 |
EDTA plasma(n=5) | 86-101 | 98 |
heparin plasma(n=5) | 93-105 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 93-101% | 92-99% | 91-99% | 79-91% |
EDTA plasma(n=5) | 99-105% | 83-103% | 83-93% | 89-98% |
heparin plasma(n=5) | 97-105% | 82-95% | 81-101% | 79-93% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Citations
- Human primary keratinocytes show a restricted ability to up-regulate suppressor of cytokine signaling (SOCS)3 compared to autologous macrophagesJcb: 280370
- Human Primary Keratinocytes Show Restricted Ability to Up-regulate Suppressor of Cytokine Signaling (SOCS)3 Protein Compared with Autologous MacrophagesPubMed: PMC3323061
- What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?Pubmed: 24368037
- Alveolar Epithelial Cell–Derived Prostaglandin E2 Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate InflammationPubmed:27183597
- The FXR Agonist, Obeticholic Acid, Suppresses HCC Proliferation & Metastasis: Role of IL-6/STAT3 Signalling Pathwaypubmed:28970500
- Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs.pubmed:29154772
- The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertilityPubmed:29914245
- Novel trajectories of Bromocriptine antidiabetic action: Leptin-IL-6/JAK2/p-STAT3/SOCS3, p-IR/p-AKT/GLUT4, PPARγ/Adiponectin, Nrf2/PARP-1, and GLP-1articles:10.3389
- Alveolar macrophage secretion of vesicular SOCS3 represents a platform for lung cancer therapeuticsPubmed: 31619584
- Olmesartan attenuates type 2 diabetes-associated liver injury: Cross-talk of AGE/RAGE/JNK, STAT3/SCOS3 and RAS signaling pathwaysPubmed: 32067934
- Resident alveolar macrophage‐derived vesicular SOCS3 dampens allergic airway inflammationPubmed: 32030817
- SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infectionPubmed: 32645550
- Changes and clinical significance of serum inflammatory factors in the treatment of pregnancy hypertension syndrome with magnesium sulfate combined with …Pubmed: 32742411
- Modulation of the Nitric Oxide/BH4 Pathway Protects Against Irradiation-Induced Neuronal Damage33755856
- Potential contribution of ELISA and LFI Assays to assessment of the oxidative stress condition based on 8-oxodG biomarker33957564