Mini Samples ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) Homo sapiens (Human) Mini ELISA

VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

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  • Mini Samples ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) Packages (Simulation)
  • Mini Samples ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) Packages (Simulation)
  • Mini Samples ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) Results demonstration
  • MEA143Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Vascular Endothelial Growth Factor A (VEGFA) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Vascular Endothelial Growth Factor A (VEGFA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-99 92
EDTA plasma(n=5) 78-102 91
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Vascular Endothelial Growth Factor A (VEGFA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-105% 84-93% 80-104% 99-105%
EDTA plasma(n=5) 88-96% 86-102% 80-101% 91-101%
heparin plasma(n=5) 84-95% 97-104% 78-104% 90-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Mini Samples ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Vascular Endothelial Growth Factor A (VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Vascular Endothelial Growth Factor A (VEGFA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Vascular Endothelial Growth Factor A (VEGFA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

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  • Can the vascular endothelial growth factor (VEGF) and total estradiol 17β be used as a marker of equine oocyte maturation?Tubitak: source
  • The correlation between resting serum leptin and serum angiogenic indices at rest and after submaximal exerciseScienceDirect: S016701151100156X
  • Multiple release of polyplexes of plasmids VEGF and bFGF from electrospun fibrous scaffolds towards regeneration of mature blood vesselsPubMed: 22484697
  • Elevated PLGF contributes to small-cell lung cancer brain metastasisPubMed: 22797069
  • Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with PolyangiitisPubMed: 22792461
  • The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary MenZjrms: Source
  • Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart.PubMed: 22827927
  • BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopausePubMed: PMC3640998
  • Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome SequencingPubMed: PMC3378547
  • A bone-seeking clone exhibits different biological properties from the ACHN parental human renal cell carcinoma in vivo and in vitroPubMed: PMC3583590
  • Intertissue Flow of Glutathione (GSH) as a Tumor Growth-promoting Mechanism: INTERLEUKIN 6 INDUCES GSH RELEASE FROM HEPATOCYTES IN METASTATIC B16 MELANOMA-BEARING MICEPubMed: PMC3091180
  • Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral MusclesNCBI:PMC3856104
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  • Prodrug of green tea epigallocatechin-3-gallate (Pro-EGCG) as a potent anti-angiogenesis agent for endometriosis in micePubmed: 22948799
  • Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cellsPubmed: 24780853
  • Biglycan up-regulated vascular endothelial growth factor (VEGF) expression and promoted angiogenesis in colon cancerPubmed:25371074
  • Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryosPubmed:25530495
  • Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In VitroPubmed:25767513
  • A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary MenPhypha:Source
  • The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cellPubMed: 26628972
  • Application of recombinant peroxisome proliferatorPubMed: 26676891
  • Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor ImmunityPubMed: 25962106
  • Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndromePubMed: 26125086
  • Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsisPubMed: 25700221
  • Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cellsPubMed: 26254457
  • Silencing of Livin inhibits tumorigenesis and metastasis via VEGF and MMPs pathway in lung cancerPubMed: 26094984
  • Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture studyPubMed: 26689470
  • Healing Mechanism and Osteogenic Capacity of Bovine Bone Mineral—Human Amniotic Mesenchymal Stem Celland Autogenous Bone Graft in Critical Size Mandibular DefectJournal: 60629
  • Immunoexpression of TGF-β/Smad and VEGF-A proteins in serum and BAL fluid of sarcoidosis patientsPubMed: 26445225
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  • ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial CellsPubmed:27075970
  • Pharmacokinetic and pharmacodynamic study of a phospholipid-based phase separation gel for once a month administration of octreotidePubmed:27040814
  • Serum angiogenic profile in abnormal placentationPubmed:26863111
  • Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosaarticle:S1742706115301410
  • Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture studyPubmed:26689470
  • The effects of ethanol on angiogenesis after myocardial infarction, and preservation of angiogenesis with rosuvastatin after heavy drinkingPubmed:27565753
  • Insulin Like Growth Factor -1(IGF-1) Promotes Angiogenesis and Reverses Ischemia Reperfusion Induced Acute Kidney Injury in Rats: Role of VEGF and TGF-β1publication:303524932_Insulin_Like_Growth_Factor_-
  • 袖带法缺血训练对单支冠状动脉慢性完全闭塞病变患者心肌缺血及左心室功能的影响58477c05699e4.pdf
  • Effects of immobilized VEGF on endothelial progenitor cells cultured on silicon substituted and nanocrystalline hydroxyapatitesc6ra19154a
  • An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells.pubmed:28240358
  • Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trialissn:2542-3975
  • Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathwaypubmed:28651490
  • 联合检测血清血管内皮生长因子和β-HCG对早期异位妊娠的诊断意义1671-2587
  • Hyperglycemia Alters the Protein Levels of Prominin-1 and VEGFA in the Retina of Albino Ratspubmed:29076766
  • Safflower Extract and Aceglutamide Injection Promoting Recovery of Peripheral Innervations via Vascular Endothelial Growth Factor-B Signaling in Diabetic MicePMC5717862
  • Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats10.1186/s13287-017-0739-3
  • Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathwaypubmed:29097668
  • Preliminary study of the treatment effect of apatinib mesylate upon malignant ascites and its safety10.21037/tcr.2017.08.24
  • A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injurypubmed:28522195
  • Functional Characterization of VEGF- and FGF-induced Tumor Blood Vessel Models in Human Cancer Xenograftspubmed:29187438
  • The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice10.1166/jbt.2017.1652
  • Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo studyISSN:1936-2625/IJCEP0055881
  • c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancerpubmed:28573141
  • Repulsive Guidance Molecule a Inhibits Angiogenesis by Downregulating VEGF and Phosphorylated Focal Adhesion Kinase In Vitro.pubmed:29018403
  • Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab.pubmed:28982871
  • c-kit Positive Cardiac Outgrowth Cells Demonstrate Better Ability for Cardiac Recovery Against Ischemic Myopathy.pubmed:29238626
  • nRGD modified lycobetaine and octreotide combination delivery system to overcome multiple barriers and enhance anti-glioma efficacy.pubmed:28544965
  • Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis.pubmed:28959186
  • The Proteomic Landscape in the Vitreous of Patients With Age-Related and Diabetic Retinal DiseasePubmed:30025106
  • Evaluation of Detection Methods and Values of Circulating Vascular Endothelial Growth Factor in Lung CancerPubmed:29675110
  • CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular RemodelingPubmed:29984229
  • 高海拔地区新生血管性青光眼不同治疗方式的疗效及对房水中PEDF, VEGF 水平的影响2018/2/201802020.pdf
  • miRNA-185 regulates the VEGFA signaling pathway in dairy cows with retained fetal membranesPubmed:29353142
  • Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate HypoxiaPubmed:29308565
  • Proteomanalyse unverdünnter Glaskörperflüssigkeit bei Patienten mit einem Venenastverschluss10.1007:s00347-017-0469-z
  • Lisinopril inhibits nuclear transcription factor kappa B and augments sensitivity to silymarin in experimental liver fibrosisPubmed: 30243070
  • Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in …Pubmed: 30353332
  • Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor RelapseDoi: 10.4143/crt.2018.214
  • Rescuing ocular development in an anophthalmic pig by blastocyst complementationPubmed: 30446498
  • Zhongfenggao protects brain microvascular endothelial cells from oxygen-glucose deprivation/reoxygenation-induced injury by angiogenesisPubmed: 30518742
  • Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker
  • Effect of voluntary running on expression of myokines in brains of rats with depressionPubmed: 30834799
  • Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer
  • Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumorsPubmed: 30952517
  • Evaluation of pro‑and anti‑tumor effects induced by three colony‑stimulating factors, G‑CSF, GM‑CSF and M‑CSF, in bladder cancer cells: Is G‑CSF a friend of bladder …
  • Whitening effects of cosmetic formulation in the vascular component of skin pigmentationPubmed: 31074159
  • A novel xeno-free culture system for human retinal pigment epithelium cellsPubmed: 31024807
  • Mammalian Eps15 homology domain 1 potentiates angiogenesis of non-small cell lung cancer by regulating β2AR signalingPubmed: 31023336
  • Strong antitumor activity of bevacizumab and aflibercept in Neuroendocrine Carcinomas: In-depth preclinical studyPubmed: 31030198
  • A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A …Pubmed: 31020669
  • Expression of Vascular Endothelial Growth Factor in the Rat Brain after Perinatal Hypoxia and Pharmacological Correction
  • IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancerPubmed: 31173199
  • Enhanced osteogenesis and angiogenesis by PCL/chitosan/Sr-doped calcium phosphate electrospun nanocomposite membrane for guided bone regenerationPubmed: 31322979
  • 小鼠肝多房棘球蚴中血管新生与病程发展的相关性研究
  • Technical Verification and Assessment of Independent Validation of Biomarker Models for EndometriosisPubmed: 31428634
  • Cardioprotective Effect of a Virgin Olive Oil Enriched with Bioactive Compounds in Spontaneously Hypertensive RatsPubmed: 31357464
  • Associations Between Genetic Polymorphisms in the VEGFA, ACE, and SOD2 Genes and Susceptibility to Diabetic Nephropathy in the Han ChinesePubmed: 31524543
  • Tazarotene Released from Aligned Electrospun Membrane Facilitates Cutaneous Wound Healing by Promoting AngiogenesisPubmed: 31503444
  • BuZangTongLuo decoction improved hindlimb ischemia by activating angiogenesis and regulating gut microbiota in diabetic micePubmed: 31654796
  • Ginkgolide B and bilobalide promote the growth and increase β‐catenin expression in hair follicle dermal papilla cells of American minksPubmed: 31520488
  • Silicon substituted hydroxyapatite/VEGF scaffolds stimulate bone regeneration in osteoporotic sheepPubmed: 31678741
  • Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expressionPubmed: 31802473
  • Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep ApneaPubmed: 31882827
  • H19 regulates angiogenic capacity of extravillous trophoblasts by H19/miR-106a-5p/VEGFA axisPubmed: 32107606
  • Syntenin overexpression in human lung cancer tissue and serum is associated with poor prognosisPubmed: 32106836
  • Soufeng Yuchuan decoction mitigates the ovalbumin-induced lung damage in a rat model of asthmaPubmed: 32036215
  • Canagliflozin impairs blood reperfusion of ischaemic lower limb partially by inhibiting the retention and paracrine function of bone marrow derived …Pubmed: 31981975
  • Nonselective alpha-/beta-AR antagonists can inhibit pericyte proliferation, migration, and secretion in vitroPubmed: 32224529
  • Long non‑coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR‑590‑3p under hypoxic conditionsPubmed: 32323787
  • The curative effects of the traditional Chinese herbal medicine “Jinchuang ointment” on excisional woundsPubmed: 32377230
  • Cyclin G2 upregulation impairs migration, invasion, and network formation through RNF123/Dvl2/JNK signaling in the trophoblast cell line HTR8/SVneo, a possible …Pubmed: 33205477
  • Paeoniflorin accelerates foot wound healing in diabetic rats though activating the Nrf2 pathwayPubmed: 33166863
  • Maternal Serum VEGF Predicts Abnormally Invasive Placenta Better than NT-proBNP: a Multicenter Case-Control StudyPubmed: 33025531
  • Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathyPubmed: 32497630
  • Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 SignalingPubmed: 32413258
  • Characterization of Endothelial Cells Associated with Cerebral Arteriovenous MalformationPubmed: 32368063
  • Molecular mechanisms of synergistic action of Ramucirumab and Paclitaxel in Gastric Cancers cell linesPubmed: 32346056
  • Effect of Chitosan oligosaccharides on ischemic symptom and gut microbiota disbalance in mice with hindlimb ischemiaPubmed: 32475560
  • Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions?Pubmed: 32722957
  • Study of serum level of vascular endothelial growth factor in patients with hepatocellular carcinoma
  • Modulatory Effects of Taurine Combined with Letrozole on Ehrlich Ascites Carcinoma in Mice
  • Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever
  • Cell-modified bioprinted microspheres for vascular regeneration
  • Strawberry (Fragaria x ananassa) leaf extract reduces brain damage in diabetic male rats
  • Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care33469783
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  • In-vivo evaluation of alginate-pectin hydrogel film loaded with simvastatin for diabetic wound healing in Streptozotocin-induced diabetic rats33421467
  • Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ¡­33705802
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