Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) Pan-species (General) Protein, Antibody, Assaykit, Service

5HT; Serotonin; N-Methyl-Gamma

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  • Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 97-104 101
heparin plasma(n=5) 87-99 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-91% 91-101% 95-103% 78-93%
EDTA plasma(n=5) 78-89% 82-92% 81-101% 80-95%
heparin plasma(n=5) 93-101% 78-104% 94-101% 83-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:5-HT) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) and unlabeled 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) ,etc. by CBA (Cytometric Bead Array) in the sample.

Giveaways

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Citations

  • Ginsenoside rich fraction of Panax ginseng C.A. Meyer improve feeding behavior following radiation-induced pica in ratsScienceDirect: S0367326X12001153
  • Sensitization induces hypersensitivity in trigeminal nervePubMed: 23106664
  • Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in ratsPubMed: 26684638
  • Catha edulis chewing effects on treatment of paranoid schizophrenic patientsPubMed: 25926735
  • Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depressionPubmed:26773866
  • Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPEPubmed:27196063
  • Metabolic Features of Heart Failure with Different Etiology ofsp11075
  • Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migrainepubmed:27618395
  • PI3K/AKT signaling pathway activation in a rat model of migraine.pubmed:28791398
  • The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic …Pubmed:29379286
  • Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile AcidsPubmed:29853946
  • Changes in sexual performance and biochemical characterisation of functional neural regions: A study in serotonin transporter knockout male ratsPubmed: 31037750
  • Serotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic InfarctionPubmed: 31153963
  • TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway InflammationPubmed: 32117301
  • Neurotransmitter system aberrations in patients with drug addictionPubmed: 32804296
  • 重组人脑利钠肽治疗慢性肺心病急性加重期的临床效果及对5-HT 浓度的影响
  • Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome
  • Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­33762821
  • O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress33651993
  • Investigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball players34090322
  • A specific primed immune response in red palm weevil, Rhynchophorus ferrugineus, is mediated by hemocyte differentiation and phagocytosisPubmed:35245605
  • Correlation between intestinal microbiotal imbalance and 5‐HT metabolism, immune inflammation in chronic unpredictable mild stress male ratsPubmed:35535862

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