Multiplex Assay Kit for Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) 
ACV-A; Activin Beta A Beta A Homodimer
- UOM
- FOB US$ 405.00 US$ 420.00 US$ 443.00 US$ 475.00 US$ 506.00 US$ 552.00 US$ 622.00 US$ 778.00
- Quantity
Overview
Properties
- Product No.CMA001Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyReproductive scienceHormone metabolism
Sign into your account
Share a new citation as an author
Upload your experimental result
Review
Contact us
Please fill in the blank.
-
Packages (Simulation)
-
Packages (Simulation)
-
ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-98 | 95 |
| EDTA plasma(n=5) | 91-105 | 98 |
| heparin plasma(n=5) | 91-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-105% | 78-95% | 92-99% | 90-101% |
| EDTA plasma(n=5) | 86-97% | 87-94% | 80-94% | 80-91% |
| heparin plasma(n=5) | 89-99% | 84-93% | 78-96% | 90-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ACVA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) and unlabeled Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Activin A (ACVA) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte DysfunctionAhajournals: Source
- Article: Hksobf_2015_V46N2_167
- Methods for Diagnosis and Treatment of Concussion or Brain Injury2016:0103140
- Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer.pubmed:27835986
- Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling.pubmed:28032440
- Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signallingdoi:10.1111
- MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis InfectionPubmed:29661829
- Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissectionPubmed:29685513
- Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis10.1093:abbs
- The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeysPubmed:29917099
- Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity10.1016:j.fbio.2018.06.002
- Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A10.2174:2211556006666170525161225
- FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB:
- SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN10.22270:ujpr.v3i2.138
- Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention:
- Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitisPubmed:29713888
- Myocardial fibrosis in hypertrophic cardiomyopathy:
- TELLING TRANSVERSAL COMPETENCES… TO BE PROFESSIONALLY PROMOTED:
- Protein biomarker panels for detecting colorectal cancer and advanced adenoma:
- Endothelotropic activity of 4-hydroxy-3, 5-di-tret-butylcinnamic acid in the conditions of experimental cerebral ischemia:
- Silencing of the CSNK2β gene by siRNA inhibits invasiveness and growth of MDA-MB-231 cells10.1101:311092
- Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular DystrophyPubmed:29067667
- SS
- PP
- DD
- hh
- OO
- MM
- EE
- II
- BB
- TT
- NN
- LL
- JJ
- Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumorsPubmed: 30952517
- The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control studyPubmed: 31647905
- The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients
- Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status34867090