Multiplex Assay Kit for Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) Bos taurus; Bovine (Cattle) Protein, Antibody, Assaykit, Service

AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide

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  • Multiplex Assay Kit for Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-95 92
EDTA plasma(n=5) 92-101 96
heparin plasma(n=5) 89-103 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-104% 90-104% 96-105% 90-98%
EDTA plasma(n=5) 94-102% 92-101% 80-91% 88-102%
heparin plasma(n=5) 96-105% 83-103% 98-105% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Adrenomedullin (ADM) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Gingival crevicular fluid adrenomedullin level in individuals with and without diabetes mellitus type 2Wiley: Source
  • Comparison of peri-implant crevicular fluid levels of adrenomedullin and human beta defensins 1 and 2 from mandibular implants with different implant stability quotient levels in nonsmoker patientsPubmed: 23898869
  • Short-term hypoxia/reoxygenation activates the angiogenic pathway in rat caudate putamen.Pubmed: 23660671
  • DNA methylation patterns in newborns exposed to tobacco in uteroPubmed:25623364
  • Adrenomedullin—A New Marker in Febrile Neutropenia: Comparison With CRP and ProcalcitoninPubMed: 26271020
  • Angiogenesis is VEGF-independent in the aged striatum of male rats exposed to acute hypoxia.pubmed:28501895
  • Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
  • ADRENOMEDULIN DI KARSINOMA PAYUDARA DENGAN METASTASIS:
  • Composition and cardioprotective effects of Primula veris L. solid herbal extract in experimental chronic heart failureDoi: 10.1016/j.phymed.2018.09.015
  • Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
  • Clinical and experimental investigation of the nervous system of neonatal foals. Neuroendocrin transition from foetal consciousness to perinatal life
  • Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy34356497

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